Cell suitable for fermentation of a mixed sugar composition

Patent metadata

Field	Value
Publication number	US-9499841-B2
Application number	US-201514750084-A
Country	US
Kind code	B2
Filing date	Jun 25, 2015
Priority date	Apr 21, 2010
Publication date	Nov 22, 2016
Grant date	Nov 22, 2016

Abstract

The present invention relates to a cell suitable for production of one or more fermentation product from a sugar composition comprising glucose, galactose, arabinose and xylose, wherein the cell comprises two to fifteen copies of one or more xylose isomerase gene or two to fifteen copies of one or more xylose reductase and xylitol dehydrogenase, and two to ten copies of araA, araB and araD, genes, wherein these genes are integrated into the cell genome.

First claim

What is claimed is:

1. A transformed Saccharomyces cerevisiae cell suitable for producing at least one fermentation product from a sugar composition comprising glucose, galactose, xylose, arabinose and mannose, wherein said cell comprises:
(a) two to fifteen copies of at least one xylose isomerase gene or two to fifteen copies of at least one xylose reductase and xylitol dehydrogenase, and
(b) from two to ten copies of L -arabinose isomerase (araA), L -ribulokinase (araB), and L -ribulose-5-phosphate 4-epimerase (araD) genes,
wherein said genes are integrated into the cell genome,
and wherein said cell comprises a disruption or deletion of the GAL80 (transcriptional repressor) gene.

2. The yeast cell according to claim 1 , wherein said cell is capable of converting at least 90% of glucose, xylose arabinose, galactose and mannose available, into a fermentation product.

3. The yeast cell according to claim 1 , wherein said cell comprises overexpressed PPP-genes TAL1 (transaldolase), TKL1 (transketolase), RPE1 (ribulose-phosphate 3-epimerase), and RKI1 (ribulose-5-phosphate isomerase).

4. The yeast cell according to claim 1 , wherein said cell comprises a XKS1 (xylulose kinase) gene.

5. The yeast cell according to claim 1 , wherein an aldose reductase gene is deleted.

6. The yeast cell according to claim 1 , wherein all genes exogenous to said cell are integrated into the genome of said cell.

7. A yeast cell according to claim 1 , wherein genes have been introduced in said cell by introduction into a host cell:
a) a cluster comprising or consisting of said genes araA, arae and araD under control of a strong constitutive promoter
b) a cluster comprising or consisting of PPP-genes TAL1, TKL1, RPE1 and RKI1, optionally under control of a strong constitutive promoter; and deletion of an aldose reductase gene;
c) a cluster comprising or consisting of a xyIA (xylose isomerase) gene and a XKS1 gene under control of a strong constitutive promoter;
d) a construct comprising a xyIA gene under control of a strong constitutive promoter, which has an ability to integrate into the genome on multiple loci;
and adaptive evolution of the mixed sugar construct for producing said yeast cell.

8. The yeast cell according to claim 7 , wherein said cell is an inhibitor resistant cell.

9. The yeast cell according to claim 7 , wherein said cell is an industrial cell.

10. A process for producing at least one fermentation product from a sugar composition comprising glucose, galactose, arabinose and xylose, wherein said sugar composition is fermented with a yeast cell according to claim 1 .

11. The process according to claim 10 , wherein said sugar composition is produced from lignocellulosic material by:
a) pretreatment of at least one lignocellulosic material to produce pretreated lignocellulosic material;
b) enzymatic treatment of the pretreated lignocellulosic material to produce said sugar composition.

12. The process according to claim 10 , wherein said fermentation is conducted anaerobically.

13. The process according to claim 10 , wherein said fermentation product is selected from the group consisting of ethanol, n-butanol, isobutanol, lactic acid, 3-hydroxy-propionic acid, acrylic acid, acetic acid, succinic acid, fumaric acid, malic acid, itaconic acid, maleic acid, citric acid, adipic acid, an amino acid, lysine, methionine, tryptophan, threonine, and aspartic acid, 1,3-propane-diol, ethylene, glycerol, a β-lactam antibiotic and a cephalosporin, vitamins, pharmaceuticals, animal feed supplements, specialty chemicals, chemical feedstocks, plastics, solvents, fuels, including biofuels and biogas or organic polymers, and an industrial enzyme, a protease, a cellulase, an amylase, a glucanase, a lactase, a lipase, a lyase, an oxidoreductases, a transferase or a xylanase.

14. The cell according to claim 8 , wherein said cell is an industrial cell.

15. The process according to claim 11 , wherein said fermentation is conducted anaerobically.

16. The yeast cell of claim 1 , wherein said at least one xylose isomerase gene is a xyIA gene.

17. The yeast cell of claim 1 , wherein the at least one fermentation product is ethanol.

18. The process of claim 10 , wherein the at least one fermentation product is ethanol.

Assignees

• Dsm Ip Assets Bv

Inventors

• Klaassen Paul
• Gielesen Bianca Elisabeth Maria
• Van Suylekom Gijsberdina Pieternella
• Heijne Wilbert Herman Marie

Classifications

• C12N9/2468

  acting on beta-galactose-glycoside bonds, e.g. carrageenases (3.2.1.83; 3.2.1.157); beta-agarase (3.2.1.81) · CPC title

• C12P7/16

  Butanols · CPC title

• C12P7/10 Primary

  substrate containing cellulosic material · CPC title

• C12P17/10

  Nitrogen as only ring hetero atom · CPC title

• C12N9/2411

  Amylases · CPC title