Use of Cry1Da in combination with Cry1Be for management of resistant insects

Patent metadata

Field	Value
Publication number	US-9499835-B2
Application number	US-201013516665-A
Country	US
Kind code	B2
Filing date	Dec 16, 2010
Priority date	Dec 16, 2009
Publication date	Nov 22, 2016
Grant date	Nov 22, 2016

Abstract

The subject invention includes methods and plants for controlling fall army worm insects, said plants comprising a Cry1Da insecticidal protein and a Cry1Be insecticidal protein, and various combinations of other proteins comprising this pair of proteins, to delay or prevent development of resistance by the insects.

First claim

We claim:

1. A transgenic corn or soybean plant comprising DNA encoding the Cry1Da of SEQ ID NO:2, DNA encoding Cry1Be of SEQ ID NO:1, and DNA encoding Cry1Fa, and wherein said transgenic plant expresses said Cry1Be, said Cry1Da, and said Cry1Fa in amounts that are insecticidally active against a Cry resistant fall armyworm.

2. A seed of the plant of claim 1 , wherein said seed comprises the DNA encoding Cry1Da, DNA encoding Cry1Be, and the DNA encoding Cry1Fa.

3. A plurality of corn or soybean plants, comprising non-Bt refuge plants and a plurality of transgenic plants of claim 1 , wherein said refuge plants comprise less than 40% of all crop plants in said plurality of plants, and wherein said plurality of plants comprises at least one refuge plant.

4. The plurality of plants of claim 3 , wherein said refuge plants comprise less than 30% of all the crop plants in said plurality of plants.

5. The plurality of plants of claim 3 , wherein said refuge plants comprise less than 20% of all the crop plants in said plurality of plants.

6. The plurality of plants of claim 3 , wherein said refuge plants comprise less than 10% of all the crop plants in said plurality of plants.

7. The plurality of plants of claim 3 , wherein said refuge plants comprise less than 5% of all the crop plants in said plurality of plants.

8. A mixture of seeds comprising refuge seeds from non-Bt refuge plants, and a plurality of seeds of claim 2 , wherein said refuge seeds comprise less than 40% of all the seeds in the mixture.

9. The mixture of seeds of claim 8 , wherein said refuge seeds comprise less than 30% of all the seeds in the mixture.

10. The mixture of seeds of claim 8 , wherein said refuge seeds comprise less than 20% of all the seeds in the mixture.

11. The mixture of seeds of claim 8 , wherein said refuge seeds comprise less than 10% of all the seeds in the mixture.

12. The mixture of seeds of claim 8 , wherein said refuge seeds comprise less than 5% of all the seeds in the mixture.

13. A method of managing development of resistance to a Cry toxin by a fall armyworm, said method comprising planting the mixture of seeds of claim 8 .

14. The plant of claim 1 , wherein said plant is a maize plant.

15. A method of controlling a Cry protein resistant fall armyworm insect, said method comprising planting the mixture of seeds of claim 8 .

Assignees

• Meade Thomas
• Narva Kenneth
• Storer Nicholas P
• Sheets Joel J
• Woosley Aaron T
• Burton Stephanie L
• Dow Agrosciences Llc

Inventors

• Meade Thomas
• Narva Kenneth
• Storer Nicholas P
• Sheets Joel J
• Woosley Aaron T
• Burton Stephanie L

Classifications

• C07K14/325

  Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins · CPC title

• C12N15/8286 Primary

  for insect resistance · CPC title

• A01N63/02

  Human Necessities · mapped topic

• A01N65/00

  Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof (containing compounds of determined constitution A01N27/00 - A01N59/00) · CPC title

• A01N63/50

  Isolated enzymes; Isolated proteins (peptides A01N37/46) · CPC title