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Dual inducible system for the condensed single protein production system
US9499825B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9499825-B2 |
| Application number | US-201213556100-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jul 23, 2012 |
| Priority date | Jul 22, 2011 |
| Publication date | Nov 22, 2016 |
| Grant date | Nov 22, 2016 |
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Abstract
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The present invention provides a dual inducible system for single protein production, as well as a method of inducing high level protein expression using amino acids.
First claim
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What is claimed: 1. A system for expressing a single target protein in an E. coli cell comprising: (i) an isolated E. coli tryptophan auxotroph; and (ii) an expression vector comprising an isolated nucleic acid sequence encoding E. coli MazF protein with W14F and W83L substitutions, a sequence-specific mRNA endoribonuclease. 2. The system of claim 1 , further comprising an expression vector comprising an isolated nucleic acid sequence encoding a target protein. 3. The system of claim 1 , further comprising a composition suitable to induce the sequence-specific mRNA endoribonuclease. 4. The system of claim 2 further comprising a composition comprising tryptophan. 5. A system for expressing a single target protein in an E. coli cell comprising: (i) an isolated E. coli histidine auxotroph; and (ii) an expression vector comprising an isolated nucleic acid sequence encoding E. coli MazF protein with G27K and H28R substitutions, a sequence-specific mRNA endoribonuclease. 6. The system of claim 5 , further comprising an expression vector comprising an isolated nucleic acid sequence encoding a target protein. 7. The system of claim 5 , further comprising a composition suitable to induce the sequence-specific mRNA endoribonuclease. 8. The system of claim 7 , further comprising a composition comprising histidine.
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Frequently asked questions
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- What does patent US9499825B2 cover?
- The present invention provides a dual inducible system for single protein production, as well as a method of inducing high level protein expression using amino acids.
- Who is the assignee on this patent?
- Inouye Masayori, Mao Lili, Vaiphei S Thangminlal, and 2 more
- What technology area does this patent fall under?
- Primary CPC classification C12N15/70. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Nov 22 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).