Microscopy imaging device with advanced imaging properties

Patent metadata

Field	Value
Publication number	US-9498135-B2
Application number	US-201514884556-A
Country	US
Kind code	B2
Filing date	Oct 15, 2015
Priority date	Aug 27, 2010
Publication date	Nov 22, 2016
Grant date	Nov 22, 2016

Abstract

Systems, methods and devices are implemented for microscope imaging solutions. One embodiment of the present disclosure is directed toward an epifluorescence microscope. The microscope includes an image capture circuit including an array of optical sensor. An optical arrangement is configured to direct excitation light of less than about 1 mW to a target object in a field of view of that is at least 0.5 mm 2 and to direct epi-fluorescence emission caused by the excitation light to the array of optical sensors. The optical arrangement and array of optical sensors are each sufficiently close to the target object to provide at least 2.5 μm resolution for an image of the field of view.

First claim

What is claimed is:

1. An epifluorescence microscopy system, the system comprising:
an optical excitation arrangement comprising an objective lens configured to direct light over an area encompassed within a field of view containing an imaging target;
an imaging circuit including an optical sensor array configured to generate image data from fluorescence caused by an interaction between the directed light and the imaging target, wherein a distance between a surface of the objective lens and the optical sensor array is at most 1 inch to increase a resolution of the generated image data;
a synchronization circuit in communication with the imaging circuit and configured to interface with an external optical-data processing system that provides visual feedback of the image data; and
an optical arrangement configured to direct the fluorescence from the area through the objective lens and to the optical sensor array with sufficient intensity and focus for the image data to reach cellular level brain imaging with a working distance of at most about 250 μm.

2. The epifluorescence microscopy system of claim 1 , wherein the optical arrangement is configured to direct the fluorescence with sufficient intensity and focus for the image data to reach at least 2.5 μm resolution.

3. The epifluorescence microscopy system of claim 1 , wherein the working distance is a distance from a surface of the objective lens to the imaging target.

4. The epifluorescence microscopy system of claim 1 , wherein the objective lens is a GRIN lens.

5. The epifluorescence microscopy system of claim 1 , wherein the epifluorescence microscopy system has a dimension parallel to a focal plane of the optical arrangement that is at most 1 inch.

6. The epifluorescence microscopy system of claim 1 , wherein the field of view is at least 0.1369 mm2.

7. The epifluorescence microscopy system of claim 1 , wherein the imaging circuit includes a CMOS sensor.

8. The epifluorescence microscopy system of claim 1 , wherein the optical excitation arrangement receives the light from a light source external to the epifluorescence microscopy system.

9. The epifluorescence microscopy system of claim 8 , wherein the light source is in optical communication with the optical excitation arrangement through a fiber optic cable.

10. The epifluorescence microscopy system of claim 1 , wherein the image data of the field of view includes multiple individual capillary blood vessels with sufficient resolution to distinguish individual capillary blood vessels from one another.

11. The epifluorescence microscopy system of claim 1 , wherein the epifluorescence microscopy system observes the imaging target in an awake freely behaving animal.

12. The epifluorescence microscopy system of claim 11 , wherein the epifluorescence microscopy system observes the imaging target in the animal while the animal is engaging in locomotor activity.

Assignees

• Univ Leland Stanford Junior

Inventors

• Ghosh Kunal
• Burns Laurie
• El Gamal Abbas
• Schnitzer Mark J
• Cocker Eric
• Ho Tatt Wei

Classifications

• G01N21/6458

  Fluorescence microscopy (fluorescence microscopes per se G02B21/0076 and G02B21/16) · CPC title

• G02B21/06

  Means for illuminating specimens · CPC title

• A61B2562/04

  Arrangements of multiple sensors of the same type · CPC title

• A61B90/20

  Surgical microscopes characterised by non-optical aspects · CPC title

• A61B5/0082

  adapted for particular medical purposes · CPC title