Device and method for microscopy using light with differing physical properties

Patent metadata

Field	Value
Publication number	US-9494782-B2
Application number	US-201314389744-A
Country	US
Kind code	B2
Filing date	Apr 5, 2013
Priority date	Apr 5, 2012
Publication date	Nov 15, 2016
Grant date	Nov 15, 2016

Abstract

The invention relates to a device for microscopy, with at least one light source for providing illumination light, with a detection unit for detecting light radiated back from a sample, with a microscopy optical unit for guiding illumination light onto the sample and for guiding light radiated back from the sample in the direction of the detection unit and with, arranged in an illumination beam path, an excitation mask ( 40 ) for providing structured illumination. The device is characterized in that the excitation mask is a spatially structured filter, which is transparent to light with a first physical property and which impresses spatial structure onto light with a second physical property that is different from the first physical property. The invention moreover relates to a method for microscopy.

First claim

The invention claimed is:

1. A device for microscopy, comprising:
at least one light source to provide illuminating light, the illuminating light including light with a first physical property and light with a second physical property different from the first physical property, wherein the first physical property is a first wavelength or a first wavelength range of the light and the second physical property is a second wavelength of the light differing from the first wavelength or a second wavelength range of the light differing from the first wavelength range,
a detection unit to detect light radiated back from a specimen,
a microscope optical system to guide illuminating light onto the specimen and to guide light radiated back from the specimen in the direction of the detection unit and
an excitation mask arranged in an illumination optical path to provide a structured illumination, wherein
the excitation mask is a spatially structured filter,
which is transparent to light with the first physical property, and
which imprints a spatial structure on light with the second physical property, and wherein illuminating light with the first physical property is transmitted substantially unchanged by the excitation mask.

2. The device as defined in claim 1 , wherein the excitation mask has one-dimensional or two-dimensional structures.

3. The device as defined in claim 1 or 2 , wherein the excitation mask has chromatically adjustable liquid crystal components.

4. The device as defined in claim 1 , wherein the excitation mask is a reflection filter or a transmission filter.

5. The device as defined in claim 1 , wherein the excitation mask has a digital micromirror.

6. The device as defined in claim 1 , wherein just one light source is provided which emits both the light with the first physical property and also light with the second physical property.

7. The device as defined in claim 1 , wherein the first wavelength is greater than the second wavelength.

8. The device as defined in claim 1 , wherein at least one of the light sources is tunable.

9. The device as defined in claim 1 , wherein at least one of the light sources is broadband.

10. The device as defined in claim 1 , further comprising a filter exchange means having a plurality of different filters at at least one of the following positions: before the detection unit or after at least one of the light sources.

11. The device as defined in claim 10 , wherein the filters are color filters.

12. The device as defined in claim 10 , wherein the filters are polarization filters.

13. The device as defined in claim 11 , wherein the different color filters are arranged on the filter exchange means such that confocal information can be read after every second image.

14. The device as defined in claim 1 , wherein a beam splitter is provided before the detection unit to separate light with the first physical property and light with the second physical property.

15. The device as defined in claim 14 , wherein the beam splitter is a color beam splitter or a polarization beam splitter.

16. The device as defined in claim 1 , wherein the detection unit has at least one sensor array.

17. The device as defined in claim 1 , wherein the detection unit has at least one multi-channel sensor array.

18. A method for microscopy,
wherein a specimen is illuminated with illuminating light, the illuminating light including light with a first physical property and light with a second physical property different from the first physical property,
wherein light radiated back from the specimen is detected,
wherein the illuminating light is spatially structured with an excitation mask,
wherein a spatially structured filter is used as an excitation mask, and
wherein the filter is transparent to light with the first physical property and the filter imprints a spatial structure on light with the second physical property, wherein the spatial structure imprinted on the light with the second physical property allows a confocal image to be obtained, and wherein illuminating light with the first physical property is transmitted substantially unchanged by the excitation mask.

19. The method as defined in claim 18 , wherein a wide-field image is recorded with the light with the first physical property.

20. The method as defined in claim 18 , wherein scanning is carried out in a direction perpendicular to the specimen.

21. The method as defined in claim 18 , wherein a first region of a sensor array is used to detect light with the first physical property and a second region of the sensor array is used to detect light with the second physical property.

22. A device for microscopy, comprising:
at least one light source to provide illuminating light, the illuminating light including light with a first physical property and light with a second physical property different from the first physical property, wherein the first physical property is a first polarization of the light and the second physical property is a second polarization of the light differing from the first polarization,
a detection unit to detect light radiated back from a specimen,
a microscope optical system to guide illuminating light onto the specimen and to guide light radiated back from the specimen in the direction of the detection unit and
an excitation mask arranged in an illumination optical path to provide a structured illumination, wherein
the excitation mask is a spatially structured filter,
which is transparent to light with the first physical property, and
which imprints a spatial structure on light with the second physical property, and wherein illuminating light with the first physical property is transmitted substantially unchanged by the excitation mask.

23. The device as defined in claim 22 , wherein the excitation mask has at least one of: chromatically adjustable liquid crystal components or a digital micromirror.

24. The device as defined in claim 22 , wherein at least one of the light sources is at least one of: tunable or broadband.

Assignees

• Zeiss Carl Microscopy Gmbh

Inventors

• Langholz Nils
• Drescher Viktor
• Lippert Helmut

Classifications

• G02B21/06 Primary

  Means for illuminating specimens · CPC title

• G02B21/0032

  Optical details of illumination, e.g. light-sources, pinholes, beam splitters, slits, fibers (G02B21/0036 - G02B21/008; means for illumination of specimens in general G02B21/06) · CPC title

• G02B26/0833

  the reflecting element being a micromechanical device, e.g. a MEMS mirror, DMD (G02B26/0825 takes precedence; micromechanical devices in general B81B) · CPC title

• G02B21/36

  arranged for photographic purposes or projection purposes (G02B21/18 takes precedence){or digital imaging or video purposes including associated control and data processing arrangements (image data processing per se G06T)} · CPC title

• G02B21/365

  Control or image processing arrangements for digital or video microscopes (G02B21/361, G02B21/362 take precedence) · CPC title