Water insoluble gel composition and method for preparing same

Patent metadata

Field	Value
Publication number	US-9493636-B2
Application number	US-201214359520-A
Country	US
Kind code	B2
Filing date	Nov 21, 2012
Priority date	Nov 24, 2011
Publication date	Nov 15, 2016
Grant date	Nov 15, 2016

Abstract

The present invention provides a method for preparing a water insoluble gel composition comprising: mixing a water soluble polysaccharide, an epoxy cross-linking agent, an alkaline compound and a solvent; and drying the thus obtained mixture at 1 to 30° C. under vacuum to remove the solvent, and a water insoluble gel composition.

First claim

The invention claimed is:

1. A method for preparing a water insoluble gel composition, comprising the following steps:
mixing a water soluble polysaccharide, an epoxy cross-linking agent, an alkaline compound and water; and
drying the thus obtained mixture at 1 to 30 C under vacuum to remove the water, wherein the drying is carried out at 1-30 mmHg thereby forming the water insoluble gel composition through cross-linking between the water soluble polysaccharide and the epoxy cross-linking agent.

2. The method for preparing a water insoluble gel composition according to claim 1 , wherein the step of removing the water is carried out in such a manner that the water soluble polysaccharide is concentrated to a concentration of 50-99 wt %.

3. The method for preparing a water insoluble gel composition according to claim 1 , wherein the water soluble polysaccharide has a molecular weight of 500,000-5,000,000 Daltons.

4. The method for preparing a water insoluble gel composition according to claim 1 , wherein the water soluble polysaccharide is used in an amount of 1-100 parts by weight based on 100 parts by weight of the water.

5. The method for preparing a water insoluble gel composition according to claim 1 , wherein the water soluble polysaccharide is at least one selected from the group consisting of hyaluronic acid, hyaluronate, poly-γ-glutamic acid, poly-γ-glutaminate, agar, alginic acid, alginate, carrageenan, furcellaran, pectin, arabic gum, karaya gum, tragacanth gum, ghatti gum, guar gum, locust bean gum, psyllium seed gum, gleatin, chitin, dextran, xanthan gum, chitosan, chondroitin-4-sulfate, chondroitin-6-sulfate and starch.

6. The method for preparing a water insoluble gel composition according to claim 1 , wherein the epoxy cross-linking agent is used in an amount of 0.01-60 parts by mole per mole of the hydroxyl groups in the water soluble polysaccharide.

7. The method for preparing a water insoluble gel composition according to claim 1 , wherein the epoxy cross-linking agent is at least one selected from the group consisting of epichlorohydrin, epibromohydrin, 1,4-butandiol diglycidyl ether, ethylene glycol diglycidyl ether, 1,6-hexanediol diglycidyl ether, propylene glycol diglycidyl ether, polyethylene glycol diglycidyl ether, polypropylene glycol diglycidyl ether, polytetramethylene glycol diglycidyl ether, neopentyl glycol diglycidyl ether, polyglycerol polyglycidyl ether, diglycerol polyglycidyl ether, glycerol polyglycidyl ether, trimethlypropane polyglycidyl ether, 1,2-(bis(2,3-epoxypropoxy)ethylene, pentaerythritol polyglycidyl ether and sorbitol polyglycidyl ether.

8. The method for preparing a water insoluble gel composition according to claim 1 , wherein the alkaline compound is a compound comprising an alkali metal including sodium, potassium, calcium and lithium.

9. A water insoluble gel composition obtained by the method as defined in claim 1 .

10. The water insoluble gel composition according to claim 9 , which comprises 1-20 wt % of the water insoluble gel based on the total weight of the composition.

11. The water insoluble gel composition according to claim 9 , which is transparent when the water insoluble gel has a swelling degree of 30-200, is translucent and milky white when the water insoluble gel has a swelling degree of 10 to less than 30, and is opaque and white when the water insoluble gel has a swelling degree of 2 to less than 10.

12. The water insoluble gel composition according to claim 9 , wherein 70-99 wt % of the originally introduced composition remains as a water insoluble gel, when the composition is introduced to a phosphate buffer solution having a pH of 6.5-7.5, swelled and pressure sterilized.

13. The water insoluble gel composition according to claim 12 , which has a weight after swelling corresponding to 2-200 times of the dry weight of the water insoluble gel remaining after the pressure sterilization.

Assignees

• Ah Young Chang
• Cho Sung Yeon
• Hwang In Seok
• Park Sun Young
• Ko Hyun Ju
• Kim Hyuk
• Kwon Min Jung
• Park Won Seok
• Amorepacific Corp

Inventors

• Ah Young Chang
• Cho Sung Yeon
• Hwang In Seok
• Park Sun Young
• Ko Hyun Ju
• Kim Hyuk
• Kwon Min Jung
• Park Won Seok

Classifications

• C08B37/0072

  Hyaluronic acid, i.e. HA or hyaluronan; Derivatives thereof, e.g. crosslinked hyaluronic acid (hylan) or hyaluronates · CPC title

• A61L2400/06

  Flowable or injectable implant compositions · CPC title

• A61L15/28

  Polysaccharides or their derivatives {(A61L15/225 takes precedence)} · CPC title

• C08L5/08 Primary

  Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof · CPC title

• A61K47/36

  Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin · CPC title