Polypeptides and their use

Patent metadata

Field	Value
Publication number	US-9493513-B2
Application number	US-201214349634-A
Country	US
Kind code	B2
Filing date	Oct 24, 2012
Priority date	Oct 24, 2011
Publication date	Nov 15, 2016
Grant date	Nov 15, 2016

Abstract

The present invention provides polypeptides that bind to inorganic solid surfaces, structures comprising such polypeptides, and methods of making such structures.

First claim

We claim:

1. A polypeptide consisting of the general formula
Z1-Z2-R1-R2-R3-T-R5-R6-R7-R8-R9-R10-R11-R12   (SEQ ID NO: 1), wherein

Z1 is absent or is a molecular tag;
Z2 is absent or is any sequence of 1-88 amino acids;
R1 is selected from the group consisting of I, T, A, V, and L;
R2 is selected from the group consisting of M, Q, A, V, L, and I;
R3 is selected from the group consisting of V, S, T, I, L, and A;
R5 is selected from the group consisting of E, K, D, N, T, S and A;
R6 is selected from the group consisting of S and P;
R7 is selected from the group consisting of S and P;
R8 is selected from the group consisting of D, R, N, and A;
R9 is selected from the group consisting of W, F, F-phenyl, Amino-F, A, and Y;
R10 is selected from the group consisting of S, D, and R;
R11 is selected from the group consisting of S, D, and R; and
R12 is selected from the group consisting of W, F, A, and Y.

2. The polypeptide of claim 1 , wherein:
R1 is selected from the group consisting of I, T, A, and L;
R2 is selected from the group consisting of M, Q, and I;
R3 is selected from the group consisting of V, S, T, and A;
R5 is selected from the group consisting of E, K, D, N, and A;
R6 is selected from the group consisting of S and P;
R7 is selected from the group consisting of S and P;
R8 is selected from the group consisting of D, R, N, and A;
R9 is selected from the group consisting of W, F, F-phenyl, Amino-F, A, and Y;
R10 is selected from the group consisting of S, D, and R;
R11 is selected from the group consisting of S, D, and R; and
R12 is selected from the group consisting of W, F, A, and Y.

3. The polypeptide of claim 1 , wherein one, two, or all three of the following are true:
R1 is I;
R2 is M; and
R3 is V.

4. The polypeptide of claim 1 , wherein one, two, three, or all four of the following are true:
R5 is E;
R6 is S;
R7 is S; and
R8 is D.

5. The polypeptide of claim 1 , wherein one, two, three, or all four of the following are true:
R5 is A;
R8 is A;
R10 is D or R; and
R11 is D or R.

6. The polypeptide of claim 1 , wherein:
R1 is I;
R2 is selected from the group consisting of M and Q;
R3 is V;
R5 is selected from the group consisting of E, K, and A;
R6 is S;
R7 is S;
R8 is selected from the group consisting of D, R, and A;
R9 is selected from the group consisting of W and Y;
R10 is S;
R11 is S; and
R12 is selected from the group consisting of W and Y.

7. The polypeptide of claim 1 , wherein
R1 is selected from the group consisting of I, T, A, V, and L;
R2 is selected from the group consisting of M, Q, A, V, L, and I;
R3 is selected from the group consisting of V, S, T, I, L, and A;
R5 is A;
R6 is selected from the group consisting of S and P;
R7 is selected from the group consisting of S and P;
R8 is A;
R9 is selected from the group consisting of W, F, F-phenyl, Amino-F, and Y;
R10 is selected from the group consisting of D, and R;
R11 is selected from the group consisting of D, and R; and
R12 is selected from the group consisting of W, F, and Y.

8. The polypeptide of claim 1 , wherein
R1 is I;
R2 is M;
R3 is V;
R5 is A;
R6 is S;
R7 is S;
R8 is A;
R9 is Y;
R10 is selected from the group consisting of D, and R;
R11 is selected from the group consisting of D, and R; and
R12 is Y.

9. The polypeptide of claim 1 , wherein at least one of the following is true:
R10 is S and R11 is S;
R9 is Y and R12 is Y;
R9 is F or W and R12 is F or W; and
R10 is D or R and R11 is D or R.

10. The polypeptide of claim 1 , wherein Z2 is present, and wherein Z2 is between 1 and 5 amino acids in length.

11. The polypeptide of claim 10 , wherein Z2 is between 1 and 2 amino acids in length.

12. The polypeptide of claim 11 , wherein Z2 is selected from the group consisting of H, W, C, SS, and VV.

13. The polypeptide of claim 1 , wherein the polypeptide comprises an amino acid sequence selected from the group consisting of:

(SEQ ID NO: 5)

IMVTESSDYSSY (GrBP5) 

(SEQ ID NO: 6)

IMVTESSDASSA (GrBP5-M1)

(SEQ ID NO: 7)

IMVTESSDWSSW (GrBP5-M2);

(SEQ ID NO: 8)

IMVTKSSRFSSF (GrBP5-M3);

(SEQ ID NO: 9)

TQSTKSSRYSSY (GrBP5-M4);

(SEQ ID NO: 10)

IMVTESSRYSSY (GrBP5-M5);

(SEQ ID NO: 2)

IMVTASSAYDDY (GrBP5-M6);

(SEQ ID NO: 11)

IMVTASSAYRDY;

(SEQ ID NO: 3)

IMVTASSAYRRY (GrBP5-M8);

(SEQ ID NO: 12)

IMVTASSDYSSY (GrBP5-12);

(SEQ ID NO: 13)

HIMVTESSDYSSY (HGrBP5);

(SEQ ID NO: 14)

WIMVTESSDYSSY (WGrBP5);

(SEQ ID NO: 15)

VVIMVTESSDYSSY (VVGrBP5);

(SEQ ID NO: 16)

SSIMVTESSDYSSY (SSGrBP5);

(SEQ ID NO: 17)

LIATESSDYSSY (GrBP5 hydrophobic);

(SEQ ID NO: 18)

AQTTESSDYSSY (GrBP 5 hydrophilic);

(SEQ ID NO: 19)

IMVTASSAYSSY (GrBP 5 neutral);

(SEQ ID NO: 20)

Bio-IMVTESSDYSSY (Bio-GrBP5);

(SEQ ID NO: 21)

IMVTEPPDYSSY (Rigid GrBP5);

(SEQ ID NO: 22)

CIMVTESSDYSSY (Cys-GrBP5);

(SEQ ID NO: 23)

DOPA-IMVTESSDYSSY (DOPA-GrBP5);

(SEQ ID NO: 24)

IMVTESSD(Nonnatural F)SSY (AminoF-GrBP5);

(SEQ ID NO: 25)

IMVTESSD(F-Phenyl)SSY (F-Phenyl-GrBP5);

Assignees

• Univ Washington Through Its Center For Commercialization

Inventors

• Mehmet Sarikaya
• Hayamizu Yuhei
• So Christopher R
• Tamerler-Behar Candan

Classifications

• C07K17/14

  Peptides being immobilised on, or in, an inorganic carrier · CPC title

• C07K14/001 Primary

  by chemical synthesis · CPC title

• C07K7/08 Primary

  having 12 to 20 amino acids (gastrins C07K14/595; somatostatins C07K14/655; melanotropins C07K14/68) · CPC title