Cell culture membrane, cell culture substrate, and method for manufacturing cell culture substrate

Patent metadata

Field	Value
Publication number	US-9492842-B2
Application number	US-201214128089-A
Country	US
Kind code	B2
Filing date	Jun 22, 2012
Priority date	Jun 24, 2011
Publication date	Nov 15, 2016
Grant date	Nov 15, 2016

Abstract

Provided is: a cell culture membrane, which is free from materials derived from living organisms, can easily be industrially mass-produced, exhibits superior long-term storage properties and chemical resistance, has excellent cell adhesion properties and long-term culture properties and is capable of replicating a cell adhesion morphology that is similar to that of collagen derived from living organisms and being used for conventional cell cultivation. Also provided are a cell culture substrate, and a method for manufacturing the cell culture substrate. In the present invention, as a cell adhesion layer, a polymer membrane represented by formula (I) is formed on the base of a cell culture substrate so as to have a membrane thickness equal to or greater than 0.2 μm (in the formula, R1 and R2 represent a —(CH 2 ) n —NH 2 moiety (n is an integer of 1-10 inclusive.) or H, with at least one of R1 and R2 being a —(CH 2 ) n —NH 2 moiety. Moreover, l and m are positive integers expressing polymerization degree).

First claim

The invention claimed is:

1. A cell culture substrate comprising:
a polymer membrane, comprising a structural unit represented by the following formula (I) and formed on a base,

(in the formula, R1 and R2 represent a direct —(CH 2 ) n —NH 2 moiety (n is an integer of 1-10 inclusive) or H, with at least one of R1 and R2 in all of the structural units in the polymer being a direct —(CH 2 ) n —NH2 moiety, wherein 1 and m are positive integers expressing polymerization degree and wherein said membrane has a thickness of at least 0.2 μm.

2. The cell culture substrate of claim 1 , wherein n is 1.

3. The cell culture substrate of claim 1 , wherein said membrane has a thickness of 0.2 μm.

4. The cell culture substrate of claim 1 , wherein said membrane has a thickness of 0.3 μm.

5. The cell culture substrate of claim 1 , wherein said membrane has a thickness of 0.7 μm.

6. The cell culture substrate of claim 1 , wherein said membrane has a thickness of 1.0 μm.

7. The cell culture substrate of claim 1 , wherein said membrane has a thickness of 1.1 μm.

8. The cell culture substrate of claim 1 , wherein said membrane has a thickness of between 0.2 μm and 1.1 μm.

9. The cell culture substrate of claim 2 , wherein said membrane has a thickness of between 0.2 μm and 1.1 μm.

10. The cell culture substrate of claim 1 , wherein said base is polystyrene.

11. The cell culture substrate of claim 1 , wherein said polymer membrane is a copolymer consisting of monoaminomethylparaxylylene and paraxylylene.

Assignees

• Yoshimoto Yasuo
• Kamimae Kentaro
• Tanabe Yuki
• Oguni Taku
• Inoue Takashi
• Mochizuki Tsutomu
• Hirama Makoto
• Fujii Teruo
• Kimura Hiroshi
• Tozawa Hideto
• Kisco Ltd
• Daisankasei Co Ltd
• Univ Tokyo

Inventors

• Yoshimoto Yasuo
• Kamimae Kentaro
• Tanabe Yuki
• Oguni Taku
• Inoue Takashi
• Mochizuki Tsutomu
• Hirama Makoto
• Fujii Teruo
• Kimura Hiroshi
• Tozawa Hideto

Classifications

• C12M25/02 Primary

  Membranes; Filters (filters or filtration in general B01D24/00-B01D41/00) · CPC title

• C12N2533/30

  Synthetic polymers · CPC title

• C12M23/20

  Material Coatings (immunocoatings C12M25/00) · CPC title

• C12N5/0068

  General culture methods using substrates (for specific animal cell type C12N5/06) · CPC title

• B05D1/60 Primary

  Deposition of organic layers from vapour phase (vapour phase deposition in general C23C14/00, C23C16/00) · CPC title