Apparatus, system, and method for collecting a target material

Patent metadata

Field	Value
Publication number	US-9492819-B2
Application number	US-201514676506-A
Country	US
Kind code	B2
Filing date	Apr 1, 2015
Priority date	Nov 30, 2012
Publication date	Nov 15, 2016
Grant date	Nov 15, 2016

Abstract

This disclosure is directed to an apparatus, system and method for retrieving a target material from a suspension. A system includes a processing vessel, such as an Eppendorf tube, a syringe or a test tube, and a collector. The collector is sized and shaped to fit into a primary vessel, such as a test tube. The collector funnels the target material from the suspension through a cannula and into the processing vessel. The cannula extends into a cavity at a first end of the collector that holds the processing vessel. The collector includes a funnel at a second end in fluid communication with the cannula. In one implementation, the processing vessel includes at least one displacement fluid to be expelled, such that the at least one displacement fluid pushes the target material into the collector.

First claim

We claim:

1. A method for collecting a target material from a suspension, the method comprising the steps of:
inserting a collector into an open end of a primary vessel that contains the suspension, the collector comprising a cavity in a first end, an opening in a second end, and a cannula to extend between the opening and the cavity;
inserting a processing vessel into the cavity, the cannula to extend into the processing vessel;
adding a displacement fluid to the processing vessel, the displacement fluid having a density greater than the density of the target material; and
centrifuging the primary vessel, the collector, the displacement fluid, and the processing vessel, wherein during centrifuging, the displacement fluid flows into the primary vessel via the collector to displace the target material from the primary vessel, which flows into the processing vessel through the collector via the cannula.

2. The method of claim 1 , further comprising the step of:
separating the suspension into suspension fractions prior to inserting the collector into the primary vessel.

3. The method of claim 2 , further comprising the step of:
adding at least one delineation fluid to the primary vessel before inserting the collector to provide further separation between the target material and any non-target material.

4. The system of claim 3 , wherein the at least one delineation fluid has a density less than the non-target material and greater than the target material or a density that is greater than the non-target material and less than the target material.

5. The method of claim 2 , further comprising the step of:
removing at least a portion of a non-target material from the primary vessel after the separating step and before inserting the collector into the primary vessel.

6. The method of claim 5 , further comprising the step of:
adding a clearing fluid to the primary vessel after the removing step, the clearing fluid having a density greater than at least the target material.

7. The method of claim 6 , further comprising the step of:
adding a float to the primary vessel prior to the separating step.

8. The method of claim 7 , further comprising the step of:
forming a seal between the float and the primary vessel by clamping after the separating step, the removing step, or adding the clearing fluid.

9. The method of claim 1 , wherein the cannula is a tube, a needle, or a non-coring needle.

10. The method of claim 1 , wherein the collector further comprises a shoulder extending circumferentially around a main body to inhibit further translation of the collector into the primary vessel.

11. The method of claim 10 , wherein the collector further comprises a lock ring to be placed over the shoulder of the collector and the open end of the primary vessel to inhibit translation of the collector relative to the primary vessel.

12. The method of claim 1 , wherein the collector further comprises an extension extending away from the first end and a lid to at least temporarily seal an opening within the extension, wherein the opening is in fluid communication with the cavity at the first end.

13. The method of claim 1 , further comprising the step of:
forming a seal between the second end of the collector and an inner wall of the primary vessel to maintain a fluid-tight sealing engagement between the collector and the inner wall of the primary vessel.

14. The method of claim 13 , wherein the seal between the second end of the collector and the inner wall of the primary vessel is formed by an interference fit, by a grease, by vacuum grease, by an adhesive, by an epoxy, by bonding, by thermal bonding, by welding, by ultrasonic welding, by clamping, by an insert that fits between the second end and the inner wall of the primary vessel, by an O-ring, or by a collar.

15. The method of claim 13 , wherein the collector further comprises a canopy in the cavity during the step of inserting the collector into the primary vessel, the canopy to inhibit any fluid that is discharged through the cannula from escaping from the first end of the collector; and removing the canopy before inserting the processing vessel into the collector.

16. The method of claim 15 , wherein the collector further comprises a window within a main body to access at least a portion of the cavity.

17. The method of claim 16 , wherein the discharged fluid flows out through the window of the collector into the primary vessel and remains above the seal between the second end and the inner wall of the primary vessel.

18. The method of claim 1 , wherein the displacement fluid is selected from the group consisting of: a solution of colloidal silica particles coated with polyvinylpyrrolidone, a polysaccharide solution, iodixanol, an organic solvent, a liquid wax, an oil, a gas, olive oil, mineral oil, silicone oil, immersion oil, mineral oil, paraffin oil, silicon oil, fluorosilicone, perfluorodecalin, perfluoroperhydrophenanthrene, perfluorooctylbromide, organic solvents, 1,4-Dioxane, acetonitrile, ethyl acetate, tert-butanol, cyclohexanone, methylene chloride, tert-Amyl alcohol, tert-Butyl methyl ether, butyl acetate, hexanol, nitrobenzene, toluene, octanol, octane, propylene carbonate, tetramethylene sulfones, ionic liquids, a polymer-based solution, a surfactant, a perfluoroketone, perfluorocyclopentanone, perfluorocyclohexanone, a fluorinated ketone, a hydrofluoroether, a hydrofluorocarbon, a perfluorocarbon, a perfluoropolyether, silicon, a silicon-based liquid, phenylmethyl siloxane, and combinations thereof.

19. The method of claim 1 , wherein the processing vessel includes a plug in a closed end, the cannula to extend through the plug.

20. The method of claim 19 , wherein the plug is composed of a resealable material.

Assignees

• Rarecyte Inc

Inventors

• Campton Daniel
• Nordberg Joshua
• Quarre Steve
• Stewart David
• Seubert Ronald
• Lundt Jonathan
• U'Ren Lance

Classifications

• G01N1/34

  Purifying; Cleaning {(processes or apparatus for extracting or separating nucleic acids from biological samples C12N15/1003)} · CPC title

• Y10T436/25375

  Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.] · CPC title

• B01D21/262

  by using a centrifuge · CPC title

• B01L2300/0672

  Integrated piercing tool · CPC title

• B01L2300/0851

  Bottom walls · CPC title