DIG-10 insecticidal cry toxins

Patent metadata

Field	Value
Publication number	US-9487798-B2
Application number	US-201313897082-A
Country	US
Kind code	B2
Filing date	May 17, 2013
Priority date	Jun 16, 2009
Publication date	Nov 8, 2016
Grant date	Nov 8, 2016

Abstract

DIG-10 Cry toxins, polynucleotides encoding such toxins, use of such toxins to control pests, and transgenic plants that produce such toxins are disclosed.

First claim

The invention claimed is:

1. A transgenic plant comprising a polynucleotide comprising SEQ ID NO:1 or SEQ ID NO:3 operably linked to a promoter that is not derived from Bacillus thuringiensis.

2. A transgenic plant comprising a polypeptide comprising SEQ ID NO:5.

3. The plant of claim 2 , wherein the polypeptide has insecticidal activity against corn rootworm.

4. A method for controlling a pest population comprising contacting said population with a pesticidally effective amount of a polypeptide comprising SEQ ID NO:5.

5. A DNA construct comprising a nucleic acid encoding a polypeptide comprising SEQ ID NO:2 or SEQ ID NO:5 or an insecticidally active fragment of SEQ ID NO:2 or SEQ ID NO:5 operably linked to a promoter that is not derived from Bacillus thuringiensis and is capable of driving expression in a plant.

6. A transgenic plant that comprises a nucleic acid encoding a polypeptide comprising SEQ ID NO:2 or SEQ ID NO:5 or an insecticidally active fragment thereof of SEQ ID NO:2 or SEQ ID NO:5 operably linked to a promoter that is not derived from Bacillus thuringiensis stably incorporated into its genome.

7. A method for protecting a plant from a pest comprising introducing into said plant the construct of claim 5 .

8. The transgenic plant of claim 6 wherein said transgenic plant comprises a dsRNA for suppression of an essential gene in an insect pest.

9. The transgenic plant of claim 6 wherein said transgenic plant comprises a dsRNA for suppression of an essential gene in corn rootworm.

10. The transgenic plant of claim 9 wherein said essential gene is selected from the group consisting of vacuolar ATPase, ADP-ribosylation factor-1, actin 42A, beta-tubulin CHD3, elongation factor-1.alpha., and transcription factor IIB.

11. The DNA construct of claim 5 wherein said DNA construct further comprises a gene encoding a dsRNA for suppression of an essential gene in an insect pest.

12. The transgenic plant of claim 6 wherein a gene encoding a dsRNA for suppression of an essential gene in an insect pest is stably incorporated into its genome.

Assignees

• Dow Agrosciences Llc

Inventors

• Lira Justin M
• Narva Kenneth
• Woosley Aaron T
• Larrinua Ignacio M
• Hey Timothy D

Classifications

• C07K14/325 Primary

  Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins · CPC title

• C12N15/8286 Primary

  for insect resistance · CPC title

• A01N65/08

  Magnoliopsida [dicotyledons] · CPC title

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title