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Polypeptides having peroxygenase activity and polynucleotides encoding same
US9487761B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9487761-B2 |
| Application number | US-201214237736-A |
| Country | US |
| Kind code | B2 |
| Filing date | Aug 10, 2012 |
| Priority date | Aug 10, 2011 |
| Publication date | Nov 8, 2016 |
| Grant date | Nov 8, 2016 |
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- Title
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- Abstract
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Abstract
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The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
First claim
Opening claim text (preview).
The invention claimed is: 1. A method of producing a polypeptide having peroxygenase activity, comprising: (a) cultivating a recombinant host cell under conditions conducive for production of the polypeptide, wherein the polypeptide has at least 90% sequence identity to the mature polypeptide of SEQ ID NO: 2 and the recombinant host cell comprises a nucleic acid construct comprising a polynucleotide encoding the polypeptide, wherein the polynucleotide is operably linked to one or more control sequences that direct the production of the polypeptide in the recombinant host cell; and (b) recovering the polypeptide. 2. The method of claim 1 , wherein the polypeptide has at least 95% sequence identity to the mature polypeptide of SEQ ID NO: 2. 3. The method of claim 1 , wherein the polypeptide is encoded by a polynucleotide that hybridizes under high stringency conditions with (i) the mature polypeptide coding sequence of SEQ ID NO: 1, (ii) the cDNA sequence thereof, or (iii) the full-length complement of (i) or (ii). 4. The method of claim 1 , wherein the polypeptide is a fragment of the mature polypeptide of SEQ ID NO: 2, wherein the fragment has peroxygenase activity. 5. The method of claim 1 , wherein the polypeptide comprises the sequence of amino acids 1-259 of SEQ ID NO: 2.
Assignees
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Classifications
Chemically modified or immobilised enzymes · CPC title
containing a N-O bond, e.g. nitro (-NO2), nitroso (-NO) · CPC title
- C12N9/0065Primary
acting on hydrogen peroxide as acceptor (1.11) · CPC title
aromatic · CPC title
containing oxidase or reductase · CPC title
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Frequently asked questions
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- What does patent US9487761B2 cover?
- The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.
- Who is the assignee on this patent?
- Landvik Sara, Oestergaard Lars H, Kalum Lisbeth, and 1 more
- What technology area does this patent fall under?
- Primary CPC classification C12N9/0065. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Nov 08 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).