Cell division tracking apparatus and method of the same, and non-transitory computer readable storage medium to store a cell division tracking program

Patent metadata

Field	Value
Publication number	US-9487746-B2
Application number	US-201414588188-A
Country	US
Kind code	B2
Filing date	Dec 31, 2014
Priority date	Jul 5, 2012
Publication date	Nov 8, 2016
Grant date	Nov 8, 2016

Abstract

A cell division tracking apparatus includes a control unit, a search range setting unit, a daughter cell judgment unit. The control unit tracks division processes of the cells based on a cell image group composed of the cell images. The search range setting unit sets a search range to search for daughter cell regions corresponding to daughter cells resulting from a division of a mother cell based on the mother cell region detected by the mother cell detection unit. The daughter cell judgment unit judges whether the cell regions are the daughter cell regions based on a region in which regions of the cell overlap the search range regarding the cell images collected at and after a detection of the mother cell region.

First claim

What is claimed is:

1. A cell division tracking apparatus comprising:
an imaging device including an image sensor, which images cells; and
a system controller which controls the apparatus to:
image the cells multiple times by the imaging device, and acquire cell images and track division processes of the cells based on a cell image group composed of the cell images;
detect a cell region in each of the cell images;
detect a mother cell region corresponding to a mother cell immediately before cell division in each of the cell images;
set a search range to search for daughter cell regions corresponding to daughter cells resulting from the division of the mother cell based on the mother cell region detected by the mother cell detection unit; and
judge whether the cell regions are the daughter cell regions based on a region in which the cell regions overlap the search range regarding the cell images collected at and after a detection of the mother cell region.

2. The cell division tracking apparatus according to claim 1 , wherein the system controller controls the apparatus to judge that the cell regions are the daughter cell regions if the area of the overlapping region of the cell regions and the search range is equal to or more than a predetermined threshold.

3. The cell division tracking apparatus according to claim 1 , wherein the system controller controls the apparatus to judge whether the cell regions are the daughter cell regions based on at least one of a size of an area of an overlapping region of the cell region and the search range, a roundness of the overlapping region, and a centroid of the overlapping region.

4. The cell division tracking apparatus according to claim 1 , wherein the system controller controls the apparatus to set the search range based on a position and a size of the mother cell region detected by the mother cell detection unit.

5. The cell division tracking apparatus according to claim 1 , the system controller further controls the apparatus to:
detect a non-division-period cell region corresponding to the cells in a non-cell-division period in the cell images; and
newly set as a search range, a region obtained by excluding the non-division-period cell region from the search range set by the search range setting unit.

6. The cell division tracking apparatus according to claim 1 , the system controller further controls the apparatus to:
calculate a feature value indicating characteristics of the cell regions; and
change the search range based on the feature value.

7. The cell division tracking apparatus according to claim 6 , wherein the system controller controls the apparatus to calculate a value indicating a roundness of the cell regions as the feature value.

8. The cell division tracking apparatus according to claim 1 , wherein the cell image group is obtained by imaging that uses a bright field microscope.

9. A non-transitory computer readable storage medium to store a cell division tracking program, the cell division tracking program enabling:
an imaging function which images cells;
a control function which controls the imaging function to images the cells multiple times and acquires a cell images, and tracks division processes of the cells based on a cell image group composed of the cell images;
a cell detection function which detects a cell region which is a region showing the cells in each of the cell images;
a mother cell detection function which detects a mother cell region corresponding to a mother cell immediately before cell division in each of the cell images;
a search range setting function which sets a search range to search for daughter cell regions corresponding to daughter cells resulting from the division of the mother cell based on the mother cell region detected by the mother cell detection function; and
a daughter cell judgment function which judges whether the cell regions are the daughter cell regions based on a region in which the cell regions overlap the search range regarding the cell images collected at and after a detection of the mother cell region.

Assignees

• Olympus Corp

Inventors

• Aragaki Hideya

Classifications

• G06T7/20 Primary

  Analysis of motion (motion estimation for coding, decoding, compressing or decompressing digital video signals H04N19/43, H04N19/51) · CPC title

• C12M41/48

  Automatic or computerized control (automatic analysis G01N35/00) · CPC title

• G06T7/0012

  Biomedical image inspection · CPC title

• C12M41/46 Primary

  of cellular or enzymatic activity or functionality, e.g. cell viability · CPC title

• G06T2207/20152

  Watershed segmentation · CPC title