Micro-patterned plate composed of an array of releasable elements surrounded with solid or gel walls

Patent metadata

Field	Value
Publication number	US-9487745-B2
Application number	US-67036508-A
Country	US
Kind code	B2
Filing date	Jul 25, 2008
Priority date	Jul 27, 2007
Publication date	Nov 8, 2016
Grant date	Nov 8, 2016

Abstract

A micro-patterned plate composed of an array of releasable elements surrounded by a gel or solid wall and methods of manufacture of the micropatterned plate. The surface properties of walls can be tailored if needed to be repellent or attracting to proteins. The walls can also inhibit cell attachment. The walls enable cells or other materials to be localized to the tops of the releasable elements. The individual element in the array of releasable elements can be released from the array by a mechanical force.

First claim

What is claimed:

1. A micropatterned plate comprising:
a plate body,
an array of a plurality of elevated sites extending from a surface of the plate body and releasably attached to the surface of the plate body, wherein the plurality of elevated sites are adapted to act as a carrier for biological material of interest adhered thereto when released from the surface of the plate body, wherein the plurality of elevated sites are releasable from the surface of the plate body leaving the structure of the plate body intact when one or more of the plurality of elevated sites is released from the surface of the plate body, and
a plurality of walls separate from the plate body and extending from the surface of the plate body contiguously across the plate body in a criss-crossing configuration, wherein the plurality of walls fill cavities between each adjacent individual ones of the plurality of elevated sites in surrounding contact with individual ones of the plurality of elevated sites interposing and forming a barrier between individual ones of the plurality of elevated sites and preventing a fluid in contact with a top surface of the individual ones of the plurality of elevated sites from the cavities between each adjacent individual ones of the plurality of elevated sites, and wherein the plurality of walls are formed such that the biological material of interest adheres only to the plurality of elevated sites
and wherein the plurality of elevated sites comprise a plurality of micro pallets.

2. The micropatterned plate of claim 1 wherein the plurality of walls are solid.

3. The micropatterened plate of claim 1 wherein the plurality of walls are formed from a gel.

4. The micropatterned plate of claim 1 wherein individual ones of the plurality of elevated sites are releasable from the surface of the plate body through application of a mechanical force.

5. The micropatterned plate of claim 1 wherein the plurality of walls are configured to prevent protein adsorption and cell attachment.

6. The micropatterned plate of claim 1 wherein the plurality of walls are configured to repel protein adsorption and cell attachment.

7. The micropatterned plate of claim 1 wherein the plurality of walls comprise a first material and the plate body comprises a second material, wherein the first and second materials are different materials.

8. The micropatterned plate of claim 7 wherein the plurality of elevated sites comprise a third material, wherein the second and third materials have differing light transmittance property wherein an optical window exists between the plurality of elevated sites to allow photopolymerization of the first material to form the plurality of walls between the plurality of elevated sites.

Assignees

• Wang Yuli
• Allbritton Nancy
• Univ California

Inventors

• Wang Yuli
• Allbritton Nancy

Classifications

• C12M25/06 Primary

  Plates; Walls; Drawers; Multilayer plates · CPC title

• C12N5/00

  Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor; (plant reproduction by tissue culture techniques A01H4/00) · CPC title

• C12M1/20 Primary

  Horizontal planar fields · CPC title

• C12M3/00

  Tissue, human, animal or plant cell, or virus culture apparatus · CPC title

• C12M23/44

  Multiple separable units; Modules · CPC title