Compositions, systems, and methods for epigenetic regulation of proprotein convertase subtilisin/kexin type 9 (PCSK9) gene expression
US-12098399-B2 · Sep 24, 2024 · US
Method for reduction of 1->2 reading frame shifts
US9487576B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9487576-B2 |
| Application number | US-201414473017-A |
| Country | US |
| Kind code | B2 |
| Filing date | Aug 29, 2014 |
| Priority date | Feb 29, 2012 |
| Publication date | Nov 8, 2016 |
| Grant date | Nov 8, 2016 |
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Abstract
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Herein is reported a method for the recombinant production of a polypeptide, which comprises the dipeptide AR, characterized in that the method comprises the recovering of the polypeptide from the cells or the cultivation medium of a cultivation of a cell comprising a nucleic acid encoding the polypeptide and thereby producing the polypeptide, whereby the dipeptide AR comprised in the polypeptide is encoded by the oligonucleotide gca cgt, or the oligonucleotide gcg cgt, or the oligonucleotide gcc cgt.
First claim
Opening claim text (preview).
The invention claimed is: 1. A method for reducing polypeptide by-product formation by 1→2 frameshift in the recombinant production of a human polypeptide comprising the dipeptide AR (SEQ ID NO: 06), characterized in that the method comprises: (a) substituting in the human polypeptide-encoding nucleic acid in the dipeptide AR encoding oligonucleotide gcg agg (SEQ ID NO: 01), or gcg aga (SEQ ID NO: 02) to obtain the oligonucleotide gca cgt (SEQ ID NO: 03), or the oligonucleotide gcg cgt (SEQ ID NO: 04), or the oligonucleotide gcc cgt (SEQ ID NO: 05), thereby producing a substituted polypeptide encoding nucleic acid, (b) expressing the substituted polypeptide-encoding nucleic acid using an E. coli host/vector, and (c) recovering the polypeptide from the cells or the cultivation medium of a cultivation of a cell comprising a nucleic acid encoding the polypeptide and thereby producing the polypeptide. 2. The method according to claim 1 , characterized in that the polypeptide has an amino acid sequence selected from the group comprising SEQ ID NO: 09, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, and SEQ ID NO: 14. 3. The method according to claim 2 , characterized in that the polypeptide has the amino acid sequence of SEQ ID NO: 09 or SEQ ID NO: 11.
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- C07K14/775Primary
Apolipopeptides · CPC title
General methods for enhancing the expression · CPC title
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- What does patent US9487576B2 cover?
- Herein is reported a method for the recombinant production of a polypeptide, which comprises the dipeptide AR, characterized in that the method comprises the recovering of the polypeptide from the cells or the cultivation medium of a cultivation of a cell comprising a nucleic acid encoding the polypeptide and thereby producing the polypeptide, whereby the dipeptide AR comprised in the polypepti…
- Who is the assignee on this patent?
- Hoffmann La Roche
- What technology area does this patent fall under?
- Primary CPC classification C07K14/775. Mapped technology areas include Chemistry & Metallurgy.
- When was this patent published?
- Publication date Tue Nov 08 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).