Scale-up of microfluidic devices

Patent metadata

Field	Value
Publication number	US-9486757-B2
Application number	US-201514710223-A
Country	US
Kind code	B2
Filing date	May 12, 2015
Priority date	Mar 13, 2009
Publication date	Nov 8, 2016
Grant date	Nov 8, 2016

Abstract

Parallel uses of microfluidic methods and devices for focusing and/or forming discontinuous sections of similar or dissimilar size in a fluid are described. In some aspects, the present invention relates generally to flow-focusing-type technology, and also to microfluidics, and more particularly parallel use of microfluidic systems arranged to control a dispersed phase within a dispersant, and the size, and size distribution, of a dispersed phase in a multi-phase fluid system, and systems for delivery of fluid components to multiple such devices.

First claim

What is claimed is:

1. A method for forming droplets in a microfluidic device in parallel, comprising:
(a) providing a microfluidic device that comprises:
(i) a plurality of subject fluid channels;
(ii) a dispersing fluid distribution channel connecting a source of dispersing fluid to a plurality of dispersing fluid outlets, wherein each of the plurality of dispersing fluid outlets is directly fluidly connected to a different microfluidic interconnected region of a separate subject fluid channel; and
(iii) one or more downstream channels fluidly connected to each of the microfluidic interconnected regions;

(b) directing subject fluids through the plurality of subject fluid channels to the microfluidic interconnected regions; and
(c) directing dispersing fluid through the dispersing fluid distribution channel to the microfluidic interconnected regions fluidly coupled thereto, to form droplets of subject fluids in a stream of dispersing fluid in the one or more downstream channels.

2. The method of claim 1 , further comprising controlling a ratio of a flow rate of one or more of the subject fluids to a flow rate of the dispersing fluid within at least one of the microfluidic interconnected regions to control droplet size.

3. The method of claim 2 , wherein the ratio of the flow rate of one or more of the subject fluids to the flow rate of the dispersing fluid within at least one of the microfluidic interconnected regions is different than the ratio of the flow rate of one or more of the subject fluids to the flow rate of the dispersing fluid within at least one other of the microfluidic interconnected regions.

4. The method of claim 1 , wherein at least one of the microfluidic interconnected regions comprises a cross junction, and the dispersing fluid is directed into the at least one microfluidic interconnected region from opposing sides of the cross junction to focus flow of the subject fluids within the at least one microfluidic interconnected region.

5. The method of claim 1 , wherein the microfluidic device further comprises a subject fluid distribution channel having a plurality of subject fluid outlets, the subject fluid outlets being fluidly connected to the plurality of subject fluid channels.

6. The method of claim 1 , wherein the dispersing fluid is an oil.

7. The method of claim 1 , wherein the subject fluids are aqueous solutions.

8. The method of claim 1 , wherein the subject fluids comprise a nucleic acid.

9. The method of claim 1 , wherein the subject fluids comprise an enzyme or a cell.

10. The method of claim 1 , wherein at least one of the one or more downstream channels is fluidly connected to a droplet outlet through which the droplets exit the microfluidic device.

11. The method of claim 10 , further comprising collecting the droplets from the droplet outlet.

12. The method of claim 1 , wherein the droplets have a cross-sectional dimension of less than 50 microns.

13. The method of claim 1 , wherein the microfluidic interconnected regions occupy a different plane than a plane occupied by the dispersing fluid distribution channel.

14. A method for forming droplets in a microfluidic device in parallel, comprising:
(a) providing a microfluidic device that comprises:
(i) a plurality of subject fluid channels each directly fluidly connected to a different microfluidic interconnected region;
(ii) a source of dispersing fluid fluidly connected to each of the microfluidic interconnected regions; and
(iii) one or more downstream channels fluidly connected to each of the microfluidic interconnected regions;

(b) directing subject fluids through the plurality of subject fluid channels to the microfluidic interconnected regions; and
(c) directing dispersing fluid from the source of dispersing fluid to the microfluidic interconnected regions fluidly coupled thereto, to form droplets of subject fluids in a stream of dispersing fluid in the one or more downstream channels.

15. The method of claim 14 , wherein the dispersing fluid is an oil.

16. The method of claim 14 , wherein the subject fluids are aqueous solutions.

17. The method of claim 14 , wherein the subject fluids comprise a nucleic acid, enzyme and/or a cell.

18. The method of claim 14 , wherein at least one of the microfluidic interconnected regions comprises a cross junction, and the dispersing fluid is directed into the at least one microfluidic interconnected region from opposing sides of the cross junction to focus flow of the subject fluids within the at least one microfluidic interconnected region.

19. The method of claim 14 , wherein at least one of the one or more downstream channels is fluidly connected to a droplet outlet through which the droplets exit the microfluidic device.

20. The method of claim 19 , further comprising collecting the droplets from the droplet outlet.

21. The method of claim 14 , wherein the droplets have a cross-sectional dimension of less than 50 microns.

Assignees

• Harvard College

Inventors

• Romanowsky Mark
• Abate Adam R
• Weitz David A

Classifications

• B01J2219/00833

  Plastic · CPC title

• B01J19/0093

  Microreactors, e.g. miniaturised or microfabricated reactors (laboratory containers with capillary fluid transport in microfabricated channels or chambers B01L3/5027) · CPC title

• B01J2219/00783

  Laminate assemblies, i.e. the reactor comprising a stack of plates · CPC title

• B01J2219/00891

  Feeding or evacuation · CPC title

• B01J2219/0097

  Optical sensors · CPC title