Methods for determining developmental stage of human cumulus cells

Patent metadata

Field	Value
Publication number	US-9481863-B2
Application number	US-201214128946-A
Country	US
Kind code	B2
Filing date	Jun 29, 2012
Priority date	Jul 1, 2011
Publication date	Nov 1, 2016
Grant date	Nov 1, 2016

Abstract

The present invention relates generally to the fields of reproductive medicine. More specifically, the present invention relates to a method determining the developmental stage of human cumulus cells issues from MII oocyte.

First claim

The invention claimed is:

1. A method for implanting an embryo in a female undergoing in vitro fertilization, comprising the steps of:
a) obtaining an oocyte with its cumulus cells from said female;
b) measuring in one of said cumulus cells an expression level of:
at least five genes selected from the Table A, and
at least seven genes selected from the Table B,

c) comparing:
the expression level of said at least five genes selected from the Table A with control expression levels of said at least five genes in immature cumulus cells; and
the expression level of said at least seven genes selected from the Table B with control expression levels of said at least seven genes in mature cumulus cells,

wherein overexpression of the at least five genes selected from Table A compared to the control expression levels of said at least five genes is indicative that said cumulus cell is a mature cumulus cell, and
overexpression of the at least seven genes selected from Table B compared to the control expression levels of said at least seven genes is indicative that said cumulus cell is an immature cumulus cell;
d) fertilizing the oocyte obtained in step a) in vitro to generate an embryo when said cumulus cell is assessed as mature in step c) or when said cumulus cell is not assessed as immature in step c); and
e) implanting said embryo in said female.

2. A method for implanting an embryo in a female undergoing in vitro fertilization, comprising the steps of:
a) obtaining an oocyte with its cumulus cells from said female;
b) measuring in one of said cumulus cells, the expression level of all the 25 genes of Table A and Table B
c) comparing:
the expression level of said genes selected from the Table A with control expression levels of said genes in immature cumulus cells; and
the expression level of said genes selected from the Table B with control expression levels of said genes in mature cumulus cells,

wherein overexpression of the genes selected from Table A compared to the control expression levels of said genes is indicative that said cumulus cell is a mature cumulus cell, and
overexpression of the genes selected from Table B compared to the control expression levels of said genes is indicative that said cumulus cell is an immature cumulus cell;
d) fertilizing the oocyte obtained in step a) in vitro to generate an embryo when said cumulus cell is assessed as mature in step c) or when said cumulus cell is not assessed as immature in step c); and
e) implanting said embryo in said female.

Assignees

• Hamamah Samir
• Inserm (Institut Nat De La Santé Et De La Rech Médicale)
• Ct Hospitalier Universitaire Montpellier
• Univ De Montpellier 1

Inventors

• Hamamah Samir

Classifications

• C12N5/0605 Primary

  Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly · CPC title

• C12N5/0682 Primary

  Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells (oocytes C12N5/0609) · CPC title

• C12N2501/115

  Basic fibroblast growth factor (bFGF, FGF-2) · CPC title

• C12N2533/54

  Collagen; Gelatin · CPC title

• C12Q1/6881

  for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes · CPC title