Method for Measuring Fibroblast Growth Factor-23 and Reagent Therefor
US-2024402163-A1 · Dec 5, 2024 · US
Insulin assay
US9476891B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9476891-B2 |
| Application number | US-201013122930-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jul 21, 2010 |
| Priority date | Jul 21, 2009 |
| Publication date | Oct 25, 2016 |
| Grant date | Oct 25, 2016 |
How to read this patent
A practical reading order for non-experts. Skip the full description unless you need deep technical detail.
- Title
What the patent document calls the invention.
- Abstract
A short plain-language summary of the technical disclosure.
- Assignees and inventors
Who owns or filed the patent and who is credited as inventor.
- Key dates
Filing, priority, publication, and grant dates set the timeline.
- First independent claim
The legal scope of protection — read this for what is actually claimed.
- CPC / IPC classifications
Technology tags used to group this patent with similar filings.
- Citations and related patents
Prior art links and similar publications in this corpus.
Abstract
Official abstract text for this publication.
The present invention provides an insulin-specific assay and an assay reagent capable of sensitively and specifically assaying insulin using an antibody having a property of reacting with insulin bound to an anti-insulin antibody while not reacting with insulin not bound to an anti-insulin antibody, without being affected by proinsulin and insulin analogs.
First claim
Opening claim text (preview).
The invention claimed is: 1. An insulin assay method comprising the steps of: contacting a sample containing insulin with a first monoclonal antibody reagent and a second monoclonal antibody reagent, wherein 1) the first monoclonal antibody reagent is the monoclonal antibody 66221, which is produced by a hybridoma which has a depository accession number of FERM BP-11233, and 2) the second monoclonal antibody reagent is the monoclonal antibody 66226, which is produced by a hybridoma that has a depository accession number of FERM BP-11234, and detecting binding of the first monoclonal antibody reagent and the second monoclonal antibody reagent to insulin in the sample and thereby obtaining a measurement value which is dependent on the concentration of insulin in the sample. 2. The insulin assay method of claim 1 , wherein the first monoclonal antibody reagent and the second monoclonal antibody reagent are immobilized to latex particles and insulin is assayed by latex immunoagglutination assay. 3. The insulin assay method of claim 1 , wherein the first monoclonal antibody reagent is immobilized to a solid phase, the second monoclonal antibody reagent is labeled with a labeling material, and insulin is assayed by ELISA. 4. The insulin assay method of claim 1 , wherein the first monoclonal antibody reagent is labeled with a labeling material, the second monoclonal antibody reagent is immobilized to a solid phase, and insulin is assayed by immunochromatography.
Assignees
Inventors
Classifications
against hormones {; against hormone releasing or inhibiting factors} · CPC title
- G01N33/53Primary
Immunoassay; Biospecific binding assay; Materials therefor · CPC title
- G01N33/74Primary
involving hormones {or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors} · CPC title
Insulins · CPC title
Double or second antibody {, i.e. precipitating antibody} · CPC title
Patent family
Related publications grouped by family.
External sources
Frequently asked questions
Answers are generated from the same data shown on this page.
- What does patent US9476891B2 cover?
- The present invention provides an insulin-specific assay and an assay reagent capable of sensitively and specifically assaying insulin using an antibody having a property of reacting with insulin bound to an anti-insulin antibody while not reacting with insulin not bound to an anti-insulin antibody, without being affected by proinsulin and insulin analogs.
- Who is the assignee on this patent?
- Kondou Junichi, Shimizu Tomo, Yamamoto Mitsuaki, and 2 more
- What technology area does this patent fall under?
- Primary CPC classification G01N33/53. Mapped technology areas include Physics.
- When was this patent published?
- Publication date Tue Oct 25 2016 00:00:00 GMT+0000 (Coordinated Universal Time) (B2). Legal status and post-grant events are not shown on this page.
- What related patents are in patentsdb?
- We list 8 related publications on this page (citations in our corpus or others sharing the same primary CPC).