Making c4+ products in bacteria
US-2015376658-A1 · Dec 31, 2015 · US
US9476069B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9476069-B2 |
| Application number | US-201514860528-A |
| Country | US |
| Kind code | B2 |
| Filing date | Sep 21, 2015 |
| Priority date | Jan 15, 2010 |
| Publication date | Oct 25, 2016 |
| Grant date | Oct 25, 2016 |
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Systems and methods for cooling and processing materials are disclosed.
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What is claimed is: 1. A method comprising: pre-treating a cellulosic and/or lignocellulosic biomass material by subjecting the biomass material to a series of cooling stages, each cooling stage being configured to cool the material to a different temperature, and irradiating and/or comminuting the biomass material between the cooling stages, converting the pre-treated cellulosic and/or lignocellulosic biomass material to a product utilizing a microorganism and/or enzyme, wherein at least one of cooling and converting the biomass material is performed using a mobile processing unit, wherein at least one of the cooling stages comprises cooling the material to a temperature below the brittle point of the material. 2. The method of claim 1 further comprising grinding or comminuting the biomass material before the biomass material has been cooled, or after cooling and before converting. 3. The method of claim 1 wherein pre-treating comprises irradiating the biomass material between cooling stages. 4. The method of claim 1 wherein the cooled biomass material has a bulk density of less than about 0.8 g/cm 3 . 5. The method of claim 2 wherein grinding or comminuting is performed in a freeze grinding or freeze milling device. 6. The method of claim 1 wherein the product comprises an alcohol. 7. The method of claim 6 wherein the product comprises ethanol and/or butanol. 8. The method of claim 1 wherein the biomass material comprises cellulose and converting the cooled material comprises utilizing an enzyme to saccharify the cellulose. 9. The method of claim 8 wherein the method further comprises fermenting the product of saccharification to produce an alcohol. 10. The method of claim 1 wherein cooling and/or converting are performed while the material is in transit. 11. The method of claim 1 wherein the mobile processing unit is transportable by ship, barge, train, or truck while in use. 12. The method of claim 3 wherein irradiating is performed at least twice. 13. The method of claim 1 wherein the biomass material is cooled to successively lower temperatures at each cooling stage. 14. The method of claim 1 wherein pre-treating comprises comminuting at least twice between cooling stages, and at each subsequent comminuting step particle size of the biomass material is further reduced.
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Monosaccharides (2-ketogulonic acid C12P7/60) · CPC title
Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source · CPC title
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