Method and apparatus for detecting clots in a liquid using measured light intensity

Patent metadata

Field	Value
Publication number	US-9470674-B2
Application number	US-201414174964-A
Country	US
Kind code	B2
Filing date	Feb 7, 2014
Priority date	Feb 21, 2013
Publication date	Oct 18, 2016
Grant date	Oct 18, 2016

Abstract

A method for detecting clots in a liquid is presented. The liquid is in a sample container. Light is irradiated having a first wavelength to the sample container by a first light source at a changeable vertical irradiating position (P_ 0 to P_n) such that the light irradiated by the first light source passes through the sample container along a first measurement path. An intensity of light having the first wavelength passing along the first measurement path and exiting the sample container is measured. Clots are detected in response to the measured intensity.

First claim

I claim:

1. A method for detecting clots in serum, the serum being in a sample container, the sample container comprising a centrifuged blood sample, the blood sample being separated into the serum and at least one other component, the method comprising:
irradiating light having a first wavelength to the sample container by a first light source at a vertical irradiating position such that the light irradiated by the first light source passes through the sample container along a first measurement path;
measuring an intensity of light having the first wavelength passing along the first measurement path and exiting the sample container;
rotating the sample container and/or the first light source around a vertical axis of the sample container without changing the vertical irradiating position such that the light irradiated by the first light source passes through the sample container along a second measurement path being different from the first measurement path;
measuring an intensity of light having the first wavelength passing along the second measurement path and exiting the sample container; and
detecting clots in serum in response to the measured intensity corresponding to the first measurement path and the measured intensity corresponding to the second measurement path.

2. The method according to claim 1 , wherein clots are detected for a given vertical irradiating position if the measured intensity corresponding to the first measurement path differs from the measured intensity corresponding to the second measurement path by more than a given quantity.

3. The method according to claim 1 , wherein when moving the sample container relative to the first light source, the sample container and/or the first light source are rotated around a vertical axis of the sample container.

4. The method according to claim 1 , wherein the first wavelength ranges from 400 nm to 1200 nm.

5. The method according to claim 1 , further comprising,
changing the vertical irradiating position.

6. The method according to claim 5 , wherein repeat the irradiation of light and the measurement of the intensity of light having the first wavelength at the changed vertical irradiating position.

7. The method according to claim 5 , wherein the detection of clots further comprises detecting clots in response to the measured intensities corresponding to the first measurement paths having different vertical irradiating positions.

8. The method according to claim 5 , wherein by changing the vertical irradiating position, the sample container is inserted into a sample container carrier.

9. The method according to claim 1 , further comprising,
irradiating light to the sample container having a second wavelength at different vertical irradiating positions;
measuring an intensity of light having the second wavelength exiting the sample container at the different vertical irradiating positions; and
calculating vertical positions of the separating medium and of the at least one other component in response to the measured intensities corresponding to the second wavelength and the measured intensities corresponding to the first wavelength.

10. The method according to claim 9 , wherein the second wavelength ranges from 1300 nm to 1700 nm.

11. An apparatus to perform a method according to claim 1 , the apparatus comprising:
the first light source;
a first measuring unit to measure an intensity of light having the first wavelength and exiting the sample container; and
a computing unit to detect clots in response to the measured intensities.

12. The apparatus according to claim 11 , further comprising,
a driving unit for moving the sample container relative to the first light source.

13. The apparatus according to claim 11 , wherein the sample container comprises a centrifuged blood sample, the blood sample being separated into serum and at least one other component, wherein the serum forms the liquid, the apparatus further comprising,
a second light source to irradiate light having the second wavelength to the sample container at different vertical irradiating positions; and
a second measuring unit to measure an intensity of light having the second wavelength and exiting the sample container, wherein the computing unit calculates positions of the separating medium and of the at least one other component in response to the measured intensities corresponding to the second wavelength and the measured intensities corresponding to the first wavelength.

14. A laboratory automation system for processing components comprised in a sample container, the system comprising:
the apparatus according to claim 11 , and
at least one aliquoter unit coupled to the apparatus, wherein the aliquoter unit performs aliquoting in response to the clot detection.

Assignees

• Roche Diagnostics Operations Inc

Inventors

• Klinec Darko

Classifications

• G01N35/02

  using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations {(G01N35/0098 and G01N35/0099 take precedence)} · CPC title

• G01N35/1016

  Control of the volume dispensed or introduced · CPC title

• G01N21/13

  Moving of cuvettes or solid samples to or from the investigating station {(handling materials for automatic analysis G01N35/00)} · CPC title

• G01N21/253

  for batch operation, i.e. multisample apparatus (analytical automats G01N35/00) · CPC title

• G01N2201/0662

  Comparing measurements on two or more paths in one sample · CPC title