Transgenic corn event MON87403 and methods for detection thereof

Patent metadata

Field	Value
Publication number	US-9469880-B2
Application number	US-201414507734-A
Country	US
Kind code	B2
Filing date	Oct 6, 2014
Priority date	Oct 9, 2013
Publication date	Oct 18, 2016
Grant date	Oct 18, 2016

Abstract

The present disclosure provides a transgenic corn comprising event MON87403 that exhibits increased grain yield. The disclosure also provides cells, plant parts, seeds, plants, commodity products related to the event, and DNA molecules that are unique to the event and were created by the insertion of transgenic DNA into the genome of a corn plant. The disclosure further provides methods for detecting the presence of said corn event nucleotide sequences in a sample, probes and primers for use in detecting nucleotide sequences that are diagnostic for the presence of said corn event.

First claim

What is claimed is:

1. A recombinant DNA molecule comprising a nucleotide sequence selected from the group consisting of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, SEQ ID NO:10, and full complements thereof.

2. The recombinant DNA molecule of claim 1 , wherein said recombinant DNA molecule is formed by the junction of an inserted heterologous nucleic acid molecule and genomic DNA of a corn plant, plant cell, or seed.

3. The recombinant DNA molecule of claim 1 , wherein said DNA molecule is from a transgenic corn plant comprising event MON87403, a representative sample of seed comprising said event having been deposited as ATCC Accession No. PTA-13584.

4. The recombinant DNA molecule of claim 1 , wherein said DNA molecule is an amplicon diagnostic for the presence of DNA from transgenic corn event MON87403.

5. The recombinant DNA molecule of claim 1 , wherein said recombinant DNA molecule is in a corn plant, plant cell, seed, progeny plant, plant part, or commodity product.

6. A transgenic corn plant, seed, cell, or plant part thereof comprising the recombinant DNA molecule of claim 1 .

7. A transgenic corn plant, seed, cell, or plant part thereof, wherein said plant, seed, cell, or plant part thereof has increased yield as compared to a control corn plant, seed, cell, or plant part thereof, and wherein said corn plant, seed, cell, or plant part thereof comprises event MON87403, a representative sample of seed comprising said event having been deposited as ATCC Accession No. PTA-13584.

8. The transgenic corn plant, seed, cell, or plant part thereof of claim 6 , the genome of which produces an amplicon comprising a DNA molecule selected from the group consisting of SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID NO:7, and SEQ ID NO:10 when tested in a DNA amplification method.

9. A corn plant or seed, comprising event MON87403, a representative sample of seed comprising said event having been deposited under ATCC Accession No. PTA-13584.

10. The corn plant or seed of claim 9 , wherein said corn plant or seed is a hybrid having at least one parent comprising event MON87403.

11. A nonliving plant material comprising the recombinant DNA molecule of claim 1 .

12. A microorganism comprising the recombinant DNA molecule of claim 1 .

13. The microorganism of claim 12 , wherein said microorganism is a plant cell.

14. A method of increasing yield in a corn plant comprising:
(a) planting a transgenic corn plant or transgenic corn seed comprising event MON87403, a representative sample of seed comprising said event having been deposited as ATCC Accession No. PTA-13584; and
(b) growing said transgenic corn plant or transgenic corn seed to obtain increase in yield in said corn plant as compared to a control corn plant.

15. A method of producing a corn plant with increased yield comprising:
(a) sexually crossing a transgenic corn plant comprising event MON87403, a representative sample of seed comprising said event having been deposited as ATCC Accession No. PTA-13584 with a second corn plant, thereby producing seed;
(b) collecting said seed produced from said cross;
(c) growing said seed to produce a plurality of progeny corn plants; and
(d) selecting a progeny corn plant comprising said event MON87403 that has increased yield as compared to a control corn plant.

16. A method of producing a corn plant with increased yield comprising:
(a) selfing a transgenic corn plant comprising event MON87403, a representative sample of seed comprising said event having been deposited as ATCC Accession No. PTA-13584, thereby producing seed;
(b) collecting said seed produced from said selfing;
(c) growing said seed to produce a plurality of progeny corn plants; and
(d) selecting a progeny corn plant comprising said event MON87403 that has increased yield as compared to a control corn plant.

17. A method of producing hybrid corn seed comprising:
(a) sexually crossing a transgenic corn plant comprising event MON87403, a representative sample of seed comprising said event having been deposited as ATCC Accession No. PTA-13584 with a second corn plant, thereby producing hybrid corn seed; and (b) selecting hybrid corn seed comprising said event MON87403.

18. The recombinant DNA molecule of claim 1 , comprising the nucleotide sequence of SEQ ID NO:1.

19. The recombinant DNA molecule of claim 1 , comprising the nucleotide sequence of SEQ ID NO:3.

20. The recombinant DNA molecule of claim 1 , comprising the nucleotide sequence of SEQ ID NO:5.

21. The recombinant DNA molecule of claim 1 , comprising the nucleotide sequence of SEQ ID NO:7.

22. The recombinant DNA molecule of claim 1 , comprising the nucleotide sequence of SEQ ID NO:10.

Assignees

• Monsanto Technology Llc
• Monsanto Technology Llc

Inventors

• Adams Thomas R
• Korte John A
• Sant Anagha M
• Taylor J Philip

Classifications

• C07K14/415

  from plants · CPC title

• C12Q2600/158

  Expression markers · CPC title

• C12N15/8261

  with agronomic (input) traits, e.g. crop yield · CPC title

• C12Q2600/13

  Plant traits · CPC title

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title