Microfluidic dispenser, cartridge and analysis system for analyzing a biological sample

Patent metadata

Field	Value
Publication number	US-9469464-B2
Application number	US-201414530861-A
Country	US
Kind code	B2
Filing date	Nov 3, 2014
Priority date	May 8, 2012
Publication date	Oct 18, 2016
Grant date	Oct 18, 2016

Abstract

A dispenser for dispensing a fluid is presented. The dispenser comprises a compressible elastomeric fluid conduit having a first end and a second end for flowing the fluid from the first end to the second end, a first check valve and a second check valve distanced in the conduit for forming a fluid chamber, and an actor for compressing the fluid chamber for dispensing a portion of the fluid. At least the second check valve is a duckbill valve. The second check valve is located at the second end of the conduit. The second check valve has sealing lips that extend beyond the second end of the conduit.

First claim

We claim:

1. A cartridge for dispending a fluid, the cartridge comprising:
a reservoir for the fluid;
a dispenser for dispensing the fluid, wherein the dispenser comprises a compressible elastomeric fluid conduit having a first end and a second end for flowing the fluid from the first end to the second end, a first check valve and a second check valve distanced in the conduit for forming a fluid chamber, wherein at least the second check valve is a duckbill valve or a rotationally symmetrical valve having a circular fluid outlet, wherein the second check valve is located at the second end of the conduit, wherein the second check valve has sealing lips that extend beyond the second end of the conduit, and wherein the dispenser has its first end connected to the reservoir for enabling an inflow of the fluid from the reservoir into the fluid chamber through the first check valve;
a wall portion, wherein the fluid chamber extends along the wall portion; and
an actor for compressing the fluid chamber for dispensing a portion of the fluid, wherein the actor presses the fluid chamber against the wall portion for compressing the fluid chamber.

2. The cartridge of claim 1 , further comprises,
a biasing element for exercising a biasing force onto the actor in order to preposition the actor relative to the fluid chamber.

3. The cartridge according to claim 1 , further comprises,
a holder for holding the actor in proximity to the fluid chamber and for guiding the actor towards the fluid chamber when the actor is urged against the fluid chamber by a drive.

4. The cartridge according to claim 3 , wherein the actor has a drive coupler for releasably coupling the actor to the drive.

5. The cartridge according to claim 4 , wherein the drive coupler has a ferromagnetic element for releasably coupling the actor to the drive.

6. The cartridge according to claim 5 , wherein the ferromagnetic element is a jawed chuck, a vacuum chuck or a collet.

7. The cartridge according to claim 1 , wherein the fluid contains a reagent.

8. An analysis system for analyzing a biological sample, the analysis system comprising:
a cartridge holder for holding a plurality of the cartridges according to claim 1 ;
a receptacle holder for holding a receptacle containing the biological sample;
a robotic component for relative movement of the cartridge holder and the receptacle;
a drive for driving actors of the cartridges; and
a controller for controlling the robotic component and the drive for dispensing one or more reagents from the cartridges into the receptacle for inducing a reaction of the biological sample with the one or more reagents to perform the analysis.

9. The analysis system according to claim 8 , further comprising,
a sensor for sensing the volume of a fluid dispensed from one of the cartridges and for generating a sensor signal indicative of the volume, wherein the controller receives the sensor signal and controls the drive for dispensing a predetermined volume of the reagent from one of the plurality of cartridges.

Assignees

• Roche Diagnostics Operations Inc

Inventors

• Boehm Christoph
• Klein Timo
• Koltay Peter
• Losleben Nadine
• Spinke Juergen
• Steinert Chris

Classifications

• B01L3/527

  for a plurality of reagents · CPC title

• B01L3/523

  with means for closing or opening · CPC title

• B01L2400/0611

  duck bill valves · CPC title

• B01L2300/123

  Flexible; Elastomeric · CPC title

• B01L3/502738

  characterised by integrated valves (throttle valves in microfluidic sample containers B01L3/502746) · CPC title