Methods and apparatus for separating cells from microcarriers
US-2018142201-A1 · May 24, 2018 · US
US9468875B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9468875-B2 |
| Application number | US-201414154387-A |
| Country | US |
| Kind code | B2 |
| Filing date | Jan 14, 2014 |
| Priority date | Jan 14, 2014 |
| Publication date | Oct 18, 2016 |
| Grant date | Oct 18, 2016 |
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A fluid reservoir includes a reservoir body forming a reservoir volume and a header opening. A header seals the header opening and includes at least a heater disposed within the reservoir volume and a fluid draw conduit. A bag filter is connected to the header such that it completely encloses the heater and the fluid draw conduit such that, when fluid is drawn from the reservoir volume through the sump location, the fluid is filtered by the bag filter.
Opening claim text (preview).
We claim: 1. A fluid reservoir adapted to enclose a fluid therewithin, the fluid reservoir comprising: a reservoir body forming a reservoir volume and a header opening that is fluidly open to the reservoir volume; a header connected to the reservoir body and disposed to seal the header opening; a fluid draw conduit connected to the header and extending through the header between a sump location, the sump location being within the reservoir volume, and a fluid draw port disposed external to the reservoir body; a bag filter connected to and at least partially enclosing the header, the bag filter completely enclosing the sump location of the fluid draw conduit within the reservoir volume such that, when fluid is drawn from the reservoir volume through the sump location, said fluid is first filtered by the bag filter; and a sump device disposed around the sump location of the fluid draw conduit, wherein the sump device forms a fluid inlet, and a secondary filter disposed to filter fluid entering the fluid draw conduit through the fluid inlet of the sump device, wherein a porosity of the secondary filter is larger than a porosity of the bag filter. 2. The fluid reservoir of claim 1 , further including a fluid level sensor connected to the header and disposed within the reservoir volume, wherein the bag filter further encloses the fluid level sensor. 3. The fluid reservoir of claim 1 , further comprising a filter carrier, the filter carrier having a generally hollow cylindrical shape and disposed within the bag filter. 4. The fluid reservoir of claim 3 , wherein the filter carrier is made of a mesh material that is permeable to the fluid that may be found the reservoir volume. 5. The fluid reservoir of claim 1 , further comprising a heater connected to the header, disposed within the reservoir volume, and adapted to be immersed in the fluid and adjacent to the sump location, wherein the heater is also disposed within the bag filter. 6. The fluid reservoir of claim 5 , wherein the heater is a coolant-operated heater formed by a coolant conduit, said coolant conduit forming a coil section towards a bottom portion of the reservoir volume, wherein the coil section further surrounds the sump location of the fluid draw conduit. 7. The fluid reservoir of claim 1 , wherein the bag filter is made from a felt fabric. 8. The fluid reservoir of claim 7 , wherein the felt fabric is made of polyethylene material. 9. The fluid reservoir of claim 1 , wherein the bag filter is connected to a mounting collar, the mounting collar being connected to the header. 10. The fluid reservoir of claim 9 , wherein the mounting collar forms an alignment feature that engages a feature of the header to control an orientation of the mounting collar relative to the header. 11. The fluid reservoir of claim 9 , wherein the mounting collar has a generally cylindrical shape and forms a plurality of legs extending towards the reservoir volume along the bag filter, wherein the plurality of legs are arranged along a periphery of the mounting collar and are disposed within the bag filter for supporting the same. 12. The fluid reservoir of claim 9 , wherein the bag filter includes a collar portion at an open end thereof, the collar portion forming at least one cutout along a circular loop thereof, the loop defining a slot extending there through extending peripherally around the collar portion, the slot configured to accept therein a band of clamp for affixing the collar portion to the mounting collar. 13. A diesel exhaust fluid (DEF) reservoir for use with an exhaust after-treatment system of an internal combustion engine, the DEF reservoir comprising: a reservoir body forming a reservoir volume and a header opening that is fluidly open to the reservoir volume; a header connected to the reservoir body and disposed to seal the header opening; a heater connected to the header and disposed within the reservoir volume and adapted to be immersed in the DEF; a DEF draw conduit connected to the header and extending through the header between a sump location, the sump location being within the reservoir volume and adjacent to the heater, and a DEF draw port disposed external to the reservoir body and fluidly connected to a DEF pump; a bag filter connected to and at least partially enclosing the header, the bag filter completely enclosing the heater and the sump location of the DEF draw conduit within the reservoir volume such that, when DEF is drawn from the reservoir volume through the sump location, said DEF is first filtered by the bag filter; and a sump device disposed around the sump location of the DEF draw conduit, wherein the sump device forms a fluid inlet, and a secondary filter disposed to filter DEF entering the DEF draw conduit through the fluid inlet of the sump device, wherein a porosity of the secondary filter is larger than a porosity of the bag filter. 14. The DEF reservoir of claim 13 , further including a DEF level sensor connected to the header and disposed within the reservoir volume, wherein the bag filter further encloses the DEF level sensor. 15. The fluid DEF of claim 13 , further comprising a filter carrier, the filter carrier having a generally hollow cylindrical shape, being made of a plastic mesh material, and being disposed within the bag filter such that the bag filter retains its shape when DEF fluid is drawn from the sump location within the bag filter. 16. The DEF reservoir of claim 13 , wherein the bag filter is made from a felt fabric, the felt fabric including at least one material selected from the group consisting of Titanium, Ni—Mo—Cr—Mn—Cu—Si—Fe alloys, hastelloy c/c-276, highly alloyed austenitic Cr—Ni-steels and Cr—Ni—Mo-steel, stainless steel, Polyethylene, Polypropylene, Polyisobutylene, Perfluoroalkoxyl alkane (PFA), Polyfluoroethylene (PFE), Polyvinyldenefluoride (PVDF), Polytetrafluoroethylene (PTFE), copolymers of vinylidenefluoride, and copolymers of hexafluoropropylene.
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