Active-electrode integrated biosensor array and methods for use thereof

Patent metadata

Field	Value
Publication number	US-9465002-B2
Application number	US-201313959492-A
Country	US
Kind code	B2
Filing date	Aug 5, 2013
Priority date	Jun 20, 2012
Publication date	Oct 11, 2016
Grant date	Oct 11, 2016

Abstract

A method and device for performing DNA sequencing and extracting structural information from unknown nucleic acid strands. The device includes a microwell structure, where identical DNA strands are immobilized within the microwell structure on a surface of a micro-bead, an active electrode or a porous polymer. The device further includes a CMOS-integrated semiconductor integrated circuit, where the CMOS-integrated semiconductor integrated circuit includes metal layers on a silicon substrate, where the metal layers form an active electrode biosensor. In addition, a sensing electrode is formed by creating openings in a passivation layer of the CMOS-integrated semiconductor integrated circuit to hold a single bead, on which the DNA strands are immobilized.

First claim

The invention claimed is:

1. A biosensor, comprising:
an electrode disposed adjacent to a fluid layer having a charged species therein;
an interface of said electrode and said fluid layer characterized by a first capacitance, wherein said fluid layer characterized by an impedance; and
detection circuitry operatively coupled to said electrode, wherein said detection circuitry comprises an operational amplifier and a capacitor, wherein said capacitor is in a parallel configurati…

Assignees

• Univ Texas

Inventors

• Hassibi Arjang
• Manickam Arun
• Singh Rituraj

Classifications

• G01N27/3276 Primary

  Physics · mapped topic

• C12Q2535/122

  Chemistry & Metallurgy · mapped topic

• G01N27/27 Primary

  Physics · mapped topic

• C12Q2565/607

  Chemistry & Metallurgy · mapped topic

• C12Q1/6874

  Chemistry & Metallurgy · mapped topic