Methods of affecting nitrogen assimilation in plants

Patent metadata

Field	Value
Publication number	US-9464296-B2
Application number	US-201113044142-A
Country	US
Kind code	B2
Filing date	Mar 9, 2011
Priority date	Mar 23, 2007
Publication date	Oct 11, 2016
Grant date	Oct 11, 2016

Abstract

Provided herein are compositions and methods for producing transgenic plants. In specific embodiments, transgenic plants comprise a construct comprising a polynucleotide encoding CCA1, GLK1 or bZIP1, operably linked to a plant-specific promote, wherein the CCA1, GLK1 or bZIP1 is ectopically overexpressed in the transgenic plants, and wherein the promoter is optionally a constitutive or inducible promoter. In other embodiments, transgenic plants in which express a lower level of CCA1, GLK1 or bZIP1 are provided. Also provided herein are commercial products (e.g., pulp, paper, paper products, or lumber) derived from the transgenic plants (e.g., transgenic trees) produced using the methods provided herein.

First claim

What is claimed is:

1. A transgenic plant that exhibits increased nitrogen-assimilation capacity as compared to a wild-type plant, the transgenic plant having a first gene construct comprising a polynucleotide encoding Arabidopsis Basic Leucine Zipper 1 (bZIP1) operatively linked to a first promoter with activity in plants, and a second gene construct comprising a polynucleotide encoding Arabidopsis Golden 2-like 1 (GLK1) operatively linked to a second promoter with activity in plants, wherein the first and second promoters are optionally (a) a constitutive, tissue-specific, or inducible promoter or (b) associated with a constitutive or inducible regulatory element, and wherein the first and second promoters can be the same or different.

2. The transgenic plant of claim 1 , wherein the plant is a species of woody, ornamental, decorative, crop, cereal, fruit, or vegetable.

3. The transgenic plant of claim 1 , wherein the plant is a species of one of the following genuses: Acorus, Aegilops, Allium, Amborella, Antirrhinum, Apium, Arachis, Beta, Betula, Brassica, Capsicum, Ceratopteris, Citrus, Cryptomeria, Cycas, Descurainia, Eschscholzia, Eucalyptus, Glycine, Gossypium, Hedyotis, Helianthus, Hordeum, Ipomoea, Lactuca, Linum, Liriodendron, Lotus, Lupinus, Lycopersicon, Medicago, Mesembryanthemum, Nicotiana, Nuphar, Pennisetum, Persea, Phaseolus, Physcomitrella, Picea, Pinus, Poncirus, Populus, Prunus, Robinia, Rosa, Saccharum, Schedonorus, Secale, Sesamum, Solanum, Sorghum, Stevia, Thellungiella, Theobroma, Triphysaria, Triticum, Vitis, Zea , or Zinnia.

4. A method of producing a plant-derived commercial product using the transgenic plant according to claim 1 .

5. The method of producing a plant-derived commercial product of claim 4 , wherein said transgenic plant is a tree, and said commercial product is pulp, paper, a paper product, or lumber.

6. The method of producing a plant-derived commercial product of claim 4 , wherein said transgenic plant is tobacco, and said commercial product is a cigarette, cigar, or chewing tobacco.

7. The method of producing a plant-derived commercial product of claim 4 , wherein said transgenic plant is a crop, and said commercial product is a fruit or vegetable.

8. The method of producing a plant-derived commercial product of claim 4 , wherein said transgenic plant is a grain, and said commercial product is bread, flour, cereal, oat meal, or rice.

9. The transgenic plant of claim 1 , wherein the plant is a species of the genus Arabidopsis.

10. The transgenic plant of claim 1 , wherein the first and second promoters are constitutive promoters.

11. The transgenic plant of claim 1 , wherein the first and second promoters are inducible promoters.

12. The transgenic plant of claim 1 , wherein the first promoter is a seed-specific promoter.

13. The transgenic plant of claim 1 , wherein the second promoter is a leaf-specific promoter.

14. The transgenic plant of claim 12 , wherein the second promoter is a leaf-specific promoter.

15. The transgenic plant of claim 3 , wherein the first and second promoters are inducible promoters.

16. The transgenic plant of claim 3 , wherein the first promoter is a seed-specific promoter.

17. The transgenic plant of claim 3 , wherein the second promoter is a leaf-specific promoter.

18. The transgenic plant of claim 17 , wherein the second promoter is a leaf-specific promoter.

19. A method of producing a plant-derived commercial product using the transgenic plant according to claim 3 .

20. The method of producing a plant-derived commercial product of claim 19 , wherein said transgenic plant is a crop, and said commercial product is a fruit or vegetable.

Assignees

• Coruzzi Gloria
• Gutierrez Rodrigo A
• Nero Damion C
• Univ New York

Inventors

• Coruzzi Gloria
• Gutierrez Rodrigo A
• Nero Damion C

Classifications

• C12N15/8222

  Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation · CPC title

• C12N15/8261

  with agronomic (input) traits, e.g. crop yield · CPC title

• C12N15/8243 Primary

  involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine · CPC title

• C07K14/415

  from plants · CPC title

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title