Methods of inhibiting Alu RNA and therapeutic uses thereof

Patent metadata

Field	Value
Publication number	US-9464289-B2
Application number	US-201414323457-A
Country	US
Kind code	B2
Filing date	Jul 3, 2014
Priority date	Jun 1, 2010
Publication date	Oct 11, 2016
Grant date	Oct 11, 2016

Abstract

The presently-disclosed subject matter includes methods of identifying an Alu RNA inhibitor, and methods and compositions for inhibiting Alu RNA. Methods and compositions can be used for the treatment of geographic atrophy and other conditions of interest.

First claim

What is claimed is:

1. An isolated nucleotide molecule selected from:
a double-stranded RNA molecule that inhibits expression of Alu RNA, wherein a first strand of the double-stranded RNA comprises a sequence selected from SEQ ID NO: 1, 2, 3, 4, 5, and 6 and including about 19 to 25 nucleotides; and
a vector comprising an oligonucleotide that inhibits the expression of Alu RNA, comprising a sequence selected from SEQ ID NO: 22, 23, 24, and 25 and including about 29 to 100 nucleotides; and a vector comprising an oligonucleotide that inhibits the expression of Alu RNA, consisting of the sequence of SEQ ID NO: 26.

2. A method of protecting an RPE cell, comprising administering a nucleotide molecule of claim 1 .

Assignees

• Univ Kentucky Res Found

Inventors

• Ambati Jayakrishna

Classifications

• G01N2500/10

  involving cells · CPC title

• C12N15/113 Primary

  Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; {Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing (when used in plants C12N15/8218)} · CPC title

• C12N2320/30

  Special therapeutic applications · CPC title

• C12Q2600/136

  Screening for pharmacological compounds · CPC title

• C12Q1/6883 Primary

  for diseases caused by alterations of genetic material · CPC title