RSV mucosal vaccine

Patent metadata

Field	Value
Publication number	US-9463236-B2
Application number	US-201214241596-A
Country	US
Kind code	B2
Filing date	Aug 29, 2012
Priority date	Aug 29, 2011
Publication date	Oct 11, 2016
Grant date	Oct 11, 2016

Abstract

A composition comprising the following composition: an AD vehicle including a synthetic peptide and a lipid, wherein the synthetic peptide includes the amino acid sequence KnLm (wherein n is 4 to 8 and m is 11 to 20), a carboxylvinyl polymer and an RSV antigen. The composition has an antibody producing ability which is further higher than that of a conventional mucosal vaccine, hence capable of exerting excellent anti-virus antigen-specific IgA antibody- and IgG antibody-inducing effect in the nasal wash and the serum, respectively, even with an extremely small quantity of an RSV antigen.

First claim

The invention claimed is:

1. A composition comprising:
(a) an antigen-drug (AD) vehicle consisting of a synthetic peptide and a lipid(s), wherein the synthetic peptide consisting of the amino acid sequence KnLm (wherein n is 4 to 8 and m is 11 to 20);
(b) a carboxyvinyl polymer; and,
(c) an F protein of a Respiratory Syncytial Virus (RSV) as an antigen.

2. The composition of claim 1 , wherein the composition is a pharmaceutical composition.

3. The composition of claim 1 , wherein the composition is a mucosal vaccine.

4. The composition of claim 3 , wherein the composition is a mucosal vaccine for a transnasal administration.

5. The composition of claim 1 , wherein the synthetic peptide consists of the amino acid sequence of SEQ ID NO: 1 or 2.

6. The composition of claim 1 , wherein the lipid is at least one of phosphatidyl choline, dipalmitoylphosphatidyl choline, phosphatidyl serine, phosphatidyl glycerol, phosphatidyl inositol, phosphatidyl ethanolamine, phosphatidic acid, lauric acid, myristic acid, palmitic acid, stearic acid and oleic acid.

7. The composition of claim 6 , wherein the lipids are a mixture of dipalmitoylphosphatidyl choline, phosphatidyl glycerol and palmitic acid.

8. The composition of claim 1 , which is produced by subjecting a mixture of the RSV-F protein and the AD vehicle to freeze-dry or ultrasonic treatment, and mixing the carboxyvinyl polymer with the mixture.

Assignees

• Kido Hiroshi
• Mizuno Dai
• Ueda Hirotsugu
• Yoshikawa Koji
• Ohsumi Keisuke
• Yamamoto Nobuyuki
• Sudo Kenji
• Univ Tokushima

Inventors

• Kido Hiroshi
• Mizuno Dai
• Ueda Hirotsugu
• Yoshikawa Koji
• Ohsumi Keisuke
• Yamamoto Nobuyuki
• Sudo Kenji

Classifications

• C07K14/005

  from viruses · CPC title

• A61K2039/541

  Mucosal route · CPC title

• A61K39/155 Primary

  Paramyxoviridae, e.g. parainfluenza virus · CPC title

• A61K2039/55511

  Organic adjuvants · CPC title

• A61P31/14

  for RNA viruses · CPC title