Polypeptides having endopeptidase activity and polynucleotides encoding same

Patent metadata

Field	Value
Publication number	US-9458446-B2
Application number	US-201113821168-A
Country	US
Kind code	B2
Filing date	Sep 30, 2011
Priority date	Oct 1, 2010
Publication date	Oct 4, 2016
Grant date	Oct 4, 2016

Abstract

The present invention relates to polypeptides having endopeptidase activity and to methods of producing and using the polypeptides. The invention also relates to methods of making a food protein hydrolysate using a trypsin-like endopeptidase derived from a bacterium.

First claim

The invention claimed is:

1. A method of producing a polypeptide having endopeptidase activity, comprising:
(a) providing a recombinant host cell comprising a polynucleotide encoding a polypeptide having endopeptidase activity operably linked to one or more heterologous control sequences that direct expression of the polypeptide, wherein the polypeptide comprises an amino acid sequence that has at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 2;
(b) cultivating the recombinant host cell in a culture medium under conditions conducive for production of the polypeptide; and
(c) recovering the polypeptide from the culture medium.

2. A method of making a protein hydrolysate comprising:
(a) providing a solution comprising protein to be hydrolyzed;
(b) adding to said solution an isolated polypeptide having endopeptidase activity, and wherein the polypeptide comprises an amino acid sequence that has at least 90% sequence identity to the amino acid sequence of SEQ ID NO: 2; and
(c) obtaining the protein hydrolysate.

3. The method of claim 2 , wherein the protein to he hydrolyzed is food protein.

4. The method of claim 1 , wherein the polypeptide having endopeptidase activity has at least 95% sequence identity to the sequence of SEQ ID NO: 2.

5. The method of claim 1 , wherein the polypeptide having endopeptidase activity has at least 97% sequence identity to the sequence of SEQ ID NO: 2.

6. The method of claim 1 , wherein the polypeptide having endopeptidase activity has at least 98% sequence identity to the sequence of SEQ ID NO: 2.

7. The method of claim 1 , wherein the polypeptide having endopeptidase activity has at least 99% sequence identity to the sequence of SEQ ID NO: 2.

8. The method of claim 1 , wherein the polypeptide having endopeptidase activity comprises or consists of the amino acid sequence of SEQ ID NO: 2.

9. The method of claim 3 , wherein the solution comprising food protein is a solution comprising protein material from a plant and/or an animal.

10. The method of claim 2 , wherein the polypeptide having endopeptidase activity has at least 95% sequence identity to the sequence of SEQ ID NO: 2.

11. The method of claim 2 , wherein the polypeptide having endopeptidase activity has at least 97% sequence identity to the sequence of SEQ ID NO: 2.

12. The method of claim 2 , wherein the polypeptide having endopeptidase activity has at least 98% sequence identity to the sequence of SEQ ID NO: 2.

13. The method of claim 2 wherein the polypeptide having endopeptidase activity has at least 99% sequence identity to the sequence of SEQ ID NO: 2.

14. The method of claim 2 wherein the polypeptide having endopeptidase activity comprises or consists of the amino acid sequence of SEQ ID NO: 2.

Assignees

• Oestergaard Peter Rahbek
• Sonksen Carsten P
• Hoff Tine
• Lynglev Gitte B
• Novozymes As

Inventors

• Oestergaard Peter Rahbek
• Sonksen Carsten P
• Hoff Tine
• Lynglev Gitte B

Classifications

• A23J3/347

  of proteins from microorganisms or unicellular algae · CPC title

• A23J3/34

  using enzymes · CPC title

• C12Y302/00

  Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2) · CPC title

• C12N9/52 Primary

  derived from bacteria {or Archaea} · CPC title

• Y02P20/52

  using catalysts, e.g. selective catalysts · CPC title