Composition of matter and method for stimulating the growth of beneficial microorganisms

Patent metadata

Field	Value
Publication number	US-9458422-B2
Application number	US-86441609-A
Country	US
Kind code	B2
Filing date	Jan 26, 2009
Priority date	Jan 25, 2008
Publication date	Oct 4, 2016
Grant date	Oct 4, 2016

Abstract

The invention describes a novel composition of matter obtained from the leaves of green plants, which is useful in promoting the growth of beneficial microorganisms. Specifically, that the invention describes a hydrolysate prepared from plant leaf biomass (leaf biomass hydrolysate or ‘LBH’) which dramatically stimulates the growth of beneficial microorganisms. Use of LBH as a fermentation substrate can also stimulate rapid production of organic acids and other organic compounds. LBH can be used as a substrate to promote the fermentation-based production of biobased industrial chemicals or biofuels, LBH can be utilized as a prebiotic to promote the growth of beneficial probiotic organisms, hi addition, LBH may also be useful in stimulating the fermentation-based production of other products, examples of which include preservatives, antibiotics, antigens, vaccines, amino acids, vitamins, recombinant proteins, bioremediation treatments, and immobilized enzymes.

First claim

What is claimed is:

1. A growth medium comprising a tobacco leaf biomass hydrolysate (TLBH) wherein TLBH is a product produced from the following steps in the following chronological order:
(a) disrupting one or more tobacco leaves;
(b) sterilizing by drying the disrupted leaves to a water activity level of approximately 0.85 or lower
(c) incubating the disrupted leaves in a liquid wherein incubating comprises heating the disrupted leaves in water to a temperature of from about 60° C. to about 90° C. for a period of time of ranging from about 15 minutes to about one (1) week;
(d) filtering the liquid after incubation to remove solids, and produce a liquid comprising soluble constituents of TLBH; and
(e) placing the soluble constituents of TLBH resulting from the filtering step of (d) into a growth medium.

2. A growth medium according to claim 1 , wherein the growth medium is a powder.

3. A growth medium according to claim 1 , wherein the growth medium is a prokaryotic growth medium.

4. A growth medium according to claim 1 , wherein the growth medium is a eukaryotic growth medium.

5. A growth medium according to claim 1 , wherein at least one desirable leaf component is removed from the leaves, during or after the leaves are disrupted.

6. A growth medium according to claim 1 , wherein the filtered liquid of step (d) is dried to a powder prior to being placed into a growth medium.

7. A growth medium according to claim 1 , wherein the dried, disrupted leaves of step (b) are packaged in filter paper, ground into a powder and packaged, or packaged as disrupted leaves without either filter paper or being ground into a powder, prior to performing the incubating of step (c).

Assignees

• Lo Yangming Martin
• Yossa Irene N N
• Belson Neil A
• Univ Maryland
• Leaf Pro Llc

Inventors

• Lo Yangming Martin
• Yossa Irene N N
• Belson Neil A

Classifications

• C12N1/16 Primary

  Yeasts; Culture media therefor · CPC title

• B65B55/12

  Sterilising contents prior to, or during, packaging · CPC title

• B01D11/0288

  Applications, solvents · CPC title

• C12N1/22 Primary

  Processes using, or culture media containing, cellulose or hydrolysates thereof · CPC title

• B65B63/08

  for heating or cooling articles or materials to facilitate packaging · CPC title