System and methods for conducting in vitro experiments

Patent metadata

Field	Value
Publication number	US-9458420-B2
Application number	US-201414483883-A
Country	US
Kind code	B2
Filing date	Sep 11, 2014
Priority date	Sep 11, 2013
Publication date	Oct 4, 2016
Grant date	Oct 4, 2016

Abstract

A system and methods by which in vitro experiments directed to a subject may be managed more efficiently including the more efficient use of a nutrient solution used in certain in vitro experimentation. Certain embodiments of the system and methods may include a retainer in which the subject of the experimentation and a nutrient solution is retainable and a recycling component that facilitates the restoration or reconditioning of the nutrient solution during the period of experimentation.

First claim

What is claimed is:

1. A system for reducing waste of nutrient solution used to maintain a subject during experimentation by restoring at least one nutrient of the nutrient solution depleted during the experimentation, comprising:
a retainer including a retainer wall defining a retainer space configured to accept and retain the nutrient solution and the subject during the experimentation; and
a restoration component comprising an exchange element including a first input element extending to an output element, the exchange element further comprising a second input element located between the first input element and the output element, wherein the second input element and the output element are positioned within the retainer space, a first nutrient received through the first input element and the nutrient solution received through the second input element are mixed together within the exchange element and released through the output element without removal of the nutrient solution from the retainer.

2. The system according to claim 1 , wherein the restoration component comprises an oxygenation component configurable to increase oxygen content of the nutrient solution.

3. The system according to claim 1 , further comprising a controller element configured to permit a user to manage amounts of one or more nutrients in the nutrient solution.

4. The system according to claim 1 , wherein the retainer wall includes at least a bottom wall and a side wall, the side wall including one or more protrusion elements that facilitate circulation of the nutrient solution within the retainer.

5. The system according to claim 1 , further comprising a controller component configurable to control the amount of one or more nutrients within the nutrient solution.

6. The system according to claim 1 , further comprising a byproducts component configurable to extract byproducts from the nutrient solution generated during the experimentation.

7. The system according to claim 1 , wherein the retainer wall includes at least a bottom wall and a side wall, the bottom wall including one or more guiding components that facilitate circulation of the nutrient solution within the retainer.

Assignees

• Univ Colorado Regents

Inventors

• Dondzillo Anna
• Klug Achim
• Lei Tim C

Classifications

• C12M23/34

  Internal compartments or partitions · CPC title

• C12M21/08 Primary

  for producing artificial tissue or for ex-vivo cultivation of tissue (prostheses A61F2/00, grafts A61L27/00) · CPC title

• C12M41/34

  of gas · CPC title

• C12M23/04

  Flat or tray type, drawers (C12M23/10, C12M23/12, C12M23/16 take precedence) · CPC title

• C12M27/20

  Baffles; Ribs; Ribbons; Auger vanes · CPC title