A proppant
US-2016376496-A1 · Dec 29, 2016 · US
US9458374B2 · US · B2
| Field | Value |
|---|---|
| Publication number | US-9458374-B2 |
| Application number | US-201213718877-A |
| Country | US |
| Kind code | B2 |
| Filing date | Dec 18, 2012 |
| Priority date | Dec 18, 2012 |
| Publication date | Oct 4, 2016 |
| Grant date | Oct 4, 2016 |
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A produced water bacterial pretreatment method which includes a) optionally adjusting a pH and a temperature of a produced water containing bacteria; b) adding a cysteine protease to said produced water; c) allowing said cysteine protease to inactivate said bacteria, thus producing pretreated produced water; and d) introducing said pretreated produced water into a wellbore penetrating a subterranean formation is provided.
Opening claim text (preview).
I claim: 1. A method of treating an oilfield fluid having an initial viscosity, comprising: a) adjusting a pH and a temperature of the oilfield fluid containing bacteria; b) adding a cysteine protease to said oilfield fluid; c) allowing said cysteine protease to inactivate said bacteria, thus producing pretreated oilfield fluid; and d) introducing said pretreated oilfield fluid into a wellbore penetrating a subterranean formation. 2. The method of claim 1 , further comprising adding a biological thickening agent to said oilfield fluid. 3. The method of claim 1 , further comprising adding a biological thickening agent to said oilfield fluid before step d). 4. The method of claim 1 , wherein said cysteine protease is selected from the group consisting of caspases, cathepsins, plant cysteine proteases, stable cysteine proteases, Ataxin UIM Domains, otubain-1, otubain-2, ataxin-3, PGPEP-1, ATG4A, TEV Protease, ATG4B/Apg4b, TIN—Ag, BLMG/bleomycin hydrolase, and UCH-L1.
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