Histamine releaser contained in human sweat

Patent metadata

Field	Value
Publication number	US-9457071-B2
Application number	US-201314409730-A
Country	US
Kind code	B2
Filing date	Jun 25, 2013
Priority date	Jun 28, 2012
Publication date	Oct 4, 2016
Grant date	Oct 4, 2016

Abstract

Provided are a sweat allergy antigen, an antibody capable of binding to the antigen specifically, and others, which are produced utilizing a microorganism-originated protein that exists in sweat allergy patient in a dissolved state or a partial peptide of the protein.

First claim

The invention claimed is:

1. A method of measuring an amount of a sweat allergy antigen protein in a sample from a human, comprising
contacting the sample with an antibody that specifically binds to a sweat allergy antigen protein selected from the group consisting of:
(i) a protein consisting of an amino acid sequence having 90% or more identity to the amino acid sequence represented by SEQ ID NO: 1; and
(ii) a peptide comprising an amino acid sequence having 90% or more identity to the amino acid sequence represented by any of SEQ ID NO: 2 to 7;

detecting the antibody that binds to the sample;
contacting a standard antibody that has the same sequence as said antibody with a standard sweat allergy antigen protein selected from the group consisting of:
(iii) a protein consisting of an amino acid sequence having 90% or more identity to the amino acid sequence represented by SEQ ID NO: 1; and
(iv) a peptide comprising an amino acid sequence having 90% or more identity to the amino acid sequence represented by any of SEQ ID NO: 2 to 7;

detecting the standard antibody that binds to the standard sweat allergy antigen protein; and
determining the amount of the sweat allergy antigen protein in the sample based on results from detecting the standard antibody that binds to the standard sweat allergy antigen protein.

2. The method of claim 1 , further comprising preparing the standard sweat allergy antigen protein, wherein
the standard sweat allergy antigen protein is a protein present in a fungus body of Malassezia globosa,
the standard sweat allergy antigen protein has a molecular weight of about 30 kDa measured by SDS-PAGE, and
the standard sweat allergy antigen protein binds to (i) serum derived from a sweat allergy patient and/or (ii) smith2 antibody.

3. The method of claim 1 , further comprising manufacturing the standard sweat allergy antigen protein comprising:
culturing Malassezia globosa at pH 7 to 9; and
isolating the sweat allergy antigen protein.

4. The method of claim 1 , further comprising preparing the standard sweat allergy antigen protein, wherein
the standard sweat allergy antigen protein is a protein secreted from a fungus body of Malassezia globosa,
the standard sweat allergy antigen protein has a molecular weight of about 17 kDa measured by SDS-PAGE, and
the standard sweat allergy antigen protein binds to (i) serum derived from a sweat allergy patient and/or (ii) smith2 antibody.

5. A method of diagnosing a sweat allergy or a disease relating to a sweat allergy antigen protein, comprising
measuring an amount of a sweat allergy antigen protein in a sample from a human according to claim 1 .

6. The method of claim 1 , wherein the sweat allergy antigen protein to be measured consists of the amino acid sequence represented by SEQ ID NO: 1.

7. The method of claim 1 , wherein the sample from a human is a human sweat, human skin washings, a solution of an extract of human skin, a human serum, or a human plasma.

8. The method of claim 1 , wherein the standard sweat allergy antigen protein is (iii) the protein consisting of an amino acid sequence having 90% or more identity to the amino acid sequence represented by SEQ ID NO: 1.

9. The method of claim 1 , wherein standard sweat allergy antigen protein is a protein consisting of the amino acid sequence represented by SEQ ID NO: 1.

10. The method of claim 1 , wherein the standard sweat allergy antigen protein is peptide comprising the amino acid sequence represented by any of SEQ ID NO: 2 to 7.

11. The method of claim 1 , wherein the standard sweat allergy antigen protein is peptide consisting of the amino acid sequence represented by any of SEQ ID NO: 2 to 7.

12. The method of claim 1 , wherein the detecting the antibody is performed using Western blotting or ELISA.

Assignees

• Univ Hiroshima

Inventors

• Hide Michihiro
• Hiragun Takaaki
• Ishii Kaori
• Mihara Shoji
• Hiragun Makiko
• Schroeder Jens-M

Classifications

• A61P37/08

  Antiallergic agents (antiasthmatic agents A61P11/06; ophthalmic antiallergics A61P27/14) · CPC title

• A61P17/00

  Drugs for dermatological disorders · CPC title

• G01N33/6854 Primary

  Immunoglobulins · CPC title

• C07K14/375

  from Basidiomycetes · CPC title

• G01N33/56961

  Plant cells or fungi · CPC title