Liquid flow cells having graphene on nitride for microscopy

Patent metadata

Field	Value
Publication number	US-9449787-B2
Application number	US-201514817551-A
Country	US
Kind code	B2
Filing date	Aug 4, 2015
Priority date	Aug 7, 2014
Publication date	Sep 20, 2016
Grant date	Sep 20, 2016

Abstract

This disclosure provides systems, methods, and apparatus related to liquid flow cells for microscopy. In one aspect, a device includes a substrate having a first and a second oxide layer disposed on surfaces of the substrate. A first and a second nitride layer are disposed on the first and second oxide layers, respectively. A cavity is defined in the first oxide layer, the first nitride layer, and the substrate, with the cavity including a third nitride layer disposed on walls of the substrate and the second oxide layer that define the cavity. A channel is defined in the second oxide layer. An inlet port and an outlet port are defined in the second nitride layer and in fluid communication with the channel. A plurality of viewports is defined in the second nitride layer. A first graphene sheet is disposed on the second nitride layer covering the plurality of viewports.

First claim

What is claimed is:

1. A device comprising:
a first plate, the first plate comprising:
a first substrate, the first substrate having a first surface and a second surface;
a first oxide layer disposed on the first surface of the first substrate and a second oxide layer disposed on the second surface of the first substrate;
a first nitride layer disposed on the first oxide layer and a second nitride layer disposed on the second oxide layer;
a cavity defined in the first oxide layer, the first nitride layer, and the first substrate, the cavity including a third nitride layer disposed on walls of the first substrate and the second oxide layer that define the cavity;
a channel defined in the second oxide layer;
an inlet port and an outlet port defined in the second nitride layer in fluid communication with the channel;
a plurality of viewports defined in the second nitride layer positioned between the inlet port and the outlet port, at least some of the plurality of viewports positioned opposite the cavity; and
a first graphene sheet disposed on the second nitride layer covering the plurality of viewports.

2. The device of claim 1 , further comprising:
a second plate, the second plate comprising:
a second substrate having a first surface and a second surface;
a third oxide layer disposed on the first surface of the second substrate and a fourth oxide layer disposed on the second surface of the second substrate; and
a third nitride layer disposed on the third oxide layer and a fourth nitride layer disposed on the fourth oxide layer, wherein the second plate defines a through hole through the third nitride layer, the third oxide layer, the second substrate, the fourth oxide layer, and the fourth nitride layer; and
a polymer material joining the graphene sheet of the first plate and the fourth nitride layer of the second plate, wherein the first and the second plates are joined so that the cavity of the first plate is aligned with the though hole in the second plate.

3. The device of claim 2 , wherein the first plate and the second plate define an inlet channel and an outlet channel, wherein the inlet channel is connected to the inlet port, and wherein the outlet channel is connected to the outlet port.

4. The device of claim 2 , wherein the polymer material is about 2 microns to 8 microns thick.

5. The device of claim 2 , wherein the polymer material is disposed proximate a perimeter of the cavity in the first substrate and a perimeter of the through hole in the second plate.

6. The device of claim 2 , wherein the polymer material comprises an epoxy-based photoresist.

7. The device of claim 1 , wherein first substrate comprises silicon, wherein the first and second oxide layers comprise silicon oxide, and wherein the first, second, and third nitride layers comprise silicon nitride.

8. The device of claim 1 , wherein the graphene sheet comprises a few layers of graphene.

9. The device of claim 1 , wherein the inlet port, the outlet port, and the plurality of view ports are about 60 nanometers to 500 nanometers in diameter.

10. The device of claim 1 , wherein the first substrate is about 150 microns to 450 microns thick.

11. The device of claim 1 , wherein the first surface and the second surface have dimensions of about 2 millimeters to 8 millimeters by about 2 millimeters to 8 millimeters.

12. The device of claim 1 , further comprising:
a first electrode disposed on the second nitride layer, wherein the first electrode comprises:
a first contact pad proximate an edge of the second nitride layer,
a first probe at an edge of the inlet port, and
a first lead connecting the first contact pad and the first probe; and

a second electrode disposed on the second nitride layer, wherein the second electrode comprises:
a second contact pad proximate the edge of the second nitride layer,
a second probe at an edge of the outlet port, and
a second lead connecting the second contact pad and the second probe.

13. The device of claim 12 , wherein the first electrode and the second electrode comprise gold or platinum.

14. The device of claim 1 , wherein a second plurality of viewports is defined in the third nitride layer, further comprising:
a second graphene sheet disposed on the third nitride layer covering the second plurality of viewports.

15. A device comprising:
a first substrate, the first substrate having a first surface and a second surface;
a first nitride layer disposed on the first surface of the first substrate and a second nitride layer disposed on the second surface of the first substrate;
an oxide layer disposed on the second nitride layer;
a third nitride layer disposed on the oxide layer;
a cavity defined in the first nitride layer and the first substrate;
a channel defined in the oxide layer;
an inlet port and an outlet port defined in the third nitride layer in fluid communication with the channel;
a plurality of viewports defined in the third nitride layer positioned between the inlet port and the outlet port, at least some of the plurality of viewports positioned opposite the cavity; and
a first graphene sheet disposed on the third nitride layer covering the plurality of viewports.

16. A method comprising:
(a) forming a first oxide layer on a first surface of a first substrate and a second oxide layer on a second surface of the first substrate;
(b) depositing a first nitride layer on the first oxide layer and a second nitride layer on the second oxide layer;
(c) removing a portion of the first nitride layer and the first oxide layer;
(d) forming a cavity in the first substrate to expose the second oxide layer;
(e) depositing a third nitride layer on the first nitride layer, first substrate walls of the cavity, and the second oxide layer;
(f) forming an inlet port, an outlet port, and a plurality of viewports between the inlet port and the outlet port in the second nitride layer, at least some of the plurality of viewports positioned to be opposite the cavity;
(g) removing a portion of the second oxide layer to form a channel from the inlet port to the outlet port; and
(h) positioning a graphene sheet on the second nitride layer to cover the plurality of viewports.

17. The method of claim 16 , further comprising:
forming a first oxide layer on a first surface of a second substrate and a second oxide layer on a second surface of the second substrate;
depositing a first nitride layer on the first oxide layer of the second substrate and a second nitride layer on the second oxide layer of the second substrate;
removing a portion of the first nitride layer and the first oxide layer of the second substrate;
forming a cavity in the second substrate to expose the second oxide layer of the second substrate;
depositing a polymer material on the second nitride layer of the second substrate;
removing a portion of the polymer material, the second nitride layer of the second substrate, and the second oxide layer of the second substrate to form a through hole; and
joining the graphene sheet of the first substrate to the second nitride layer of the second substrate with the polymer material, wherein the cavity of the first substrate is aligned with the though hole of the second substrate.

18. The method of claim 16 , further comprising:
after operation (e), forming a first electrode on the second nitride layer, wherein the first electrode includes a first contact pad proximate an edge of the second nitride layer, a first probe at an edge of the inlet port, and a first lead connecting the first contact

Assignees

• Adiga Vivekananda P
• Dunn Gabriel
• Zettl Alexander K
• Alivisatos A Paul
• Univ California

Inventors

• Adiga Vivekananda P
• Dunn Gabriel
• Zettl Alexander K
• Alivisatos A Paul

Classifications

• H10D62/8303

  Diamond · CPC title

• H10D62/882

  Graphene · CPC title

• H10D30/01

  Manufacture or treatment · CPC title

• H01J37/20 Primary

  Means for supporting or positioning the object or the material; Means for adjusting diaphragms or lenses associated with the support · CPC title

• C23C18/08

  characterised by the deposition of metallic material · CPC title