Modified Bacillus thuringiensis cry proteins that inhibit coleopterans

Patent metadata

Field	Value
Publication number	US-9447153-B1
Application number	US-201213621582-A
Country	US
Kind code	B1
Filing date	Sep 17, 2012
Priority date	Jul 30, 2008
Publication date	Sep 20, 2016
Grant date	Sep 20, 2016

Abstract

The present invention includes domain I modifications that improve various attributes of various Coleopteran-active Cry proteins. These attributes can include improved target pest spectrum, potency, and insect resistance management. The subject modifications can affect protoxin activation and the efficiency of pore formation, which can lead to enhanced insect intoxication.

First claim

The invention claimed is:

1. A Cry3A protein having a protease cleavage site inserted between α-Helix2A and α-Helix2B in domain I.

2. The protein of claim 1 wherein said protein is a Cry3Aa protein.

3. A method of inhibiting an insect, said method comprising providing the protein of claim 1 to said insect for ingestion.

4. A polynucleotide encoding the protein of claim 1 .

5. A plant cell comprising the polynucleotide of claim 4 .

6. A plant comprising a plurality of the plant cell of claim 5 .

7. A method of protecting a plant from insect damage, said method comprising providing the protein of claim 1 to said insect for ingestion.

8. The method of claim 7 , wherein said protein is produced by and is present in said plant.

9. The protein of claim 1 , wherein said protease cleavage site is more susceptible to cleavage by an insect protease than by a plant protease.

Assignees

• Dow Agrosciences Llc

Inventors

• Hey Timothy D
• Larrinua Ignacio M
• Narva Kenneth E

Classifications

• A01N37/46

  N-acyl derivatives · CPC title

• C12N15/8286

  for insect resistance · CPC title

• C07K2319/50

  containing protease site · CPC title

• C07K14/325 Primary

  Bacillus thuringiensis crystal peptides, i.e. delta-endotoxins · CPC title

• A01N63/50

  Isolated enzymes; Isolated proteins (peptides A01N37/46) · CPC title