Beta sheet tapes ribbons in tissue engineering

Patent metadata

Field	Value
Publication number	US-9447151-B2
Application number	US-201615066818-A
Country	US
Kind code	B2
Filing date	Mar 10, 2016
Priority date	Jul 15, 2002
Publication date	Sep 20, 2016
Grant date	Sep 20, 2016

Abstract

There is described a material comprising tapes, ribbons, fibrils or fibers characterized in that each of the ribbons, fibrils or fibers have an antiparallel arrangement of peptides in a β-sheet tape-like substructure wherein the material comprises a pair of self assembling complementary polypeptides.

First claim

The invention claimed is:

1. A pair of self assembling complementary polypeptides (SACPs) that form tapes, ribbons, fibrils or fibres in a β-sheet tape-like substructure, when a first monomeric peptide is contacted or mixed with its complementary monomeric peptide and wherein the SACPs comprise P 11 -26/27 (SEQ ID NOs: 4 and 5).

2. The SACPs of claim 1 , further comprising a bioactive peptide or a therapeutically active molecule conjugated to the SACPs.

3. A composition, comprising:
tapes, ribbons, fibrils or fibres, wherein each of the tapes, ribbons, fibrils or fibres has an antiparallel arrangement of SACPs in a β-sheet tape-like substructure, wherein the SACPs comprise P 11 -26/27 (SEQ ID NOs: 4 and 5).

4. The composition of claim 3 , wherein the composition comprises SACPs which form ribbons and/or fibrils immediately when a first monomeric peptide contacts a second complementary monomeric peptide.

5. The composition of claim 3 , wherein the SACPs further comprise a bioactive peptide or a therapeutically active molecule conjugated to the SACPs.

6. The composition of claim 3 , wherein the composition comprises fibrils, and wherein the fibrils are comprised in a network of fibrils interconnected at fibre-like junctions.

7. The composition of claim 3 , wherein the composition comprises a dental product.

8. The composition of claim 7 , wherein the dental product is a dental repair, dental treatment, or dental reconstruction product.

9. The composition of claim 3 , wherein the composition is in the form of a tissue engineering scaffold.

10. The composition according to claim 9 , wherein the scaffold is seeded with cells.

11. The composition of claim 3 , wherein the material comprises a tissue repair product.

12. The composition of claim 3 , wherein the material comprises a wound healing product.

13. The composition of claim 3 , wherein the material comprises a skin treatment.

14. The composition of claim 3 , wherein the composition comprises a hair care product.

15. The composition of claim 3 , wherein the composition is part of a bioresponsive and biocompatible surface.

16. The composition of claim 3 , wherein the composition is a template for nucleation and growth of inorganic materials.

17. The composition of claim 3 , wherein the composition comprises a continuous filament yarn, staple, floc, cord, or fabric.

18. The composition of claim 3 , wherein the composition comprises a bone reconstruction product.

Assignees

• Univ Leeds Innovations Ltd
• The Univ Of Leeds

Inventors

• Boden Neville
• Aggeli Amalia
• Ingham Eileen
• Kirkham Jennifer

Classifications

• A61K6/884

  comprising natural or synthetic resins · CPC title

• A61K6/15

  Compositions characterised by their physical properties · CPC title

• A61K6/80

  Preparations for artificial teeth, for filling teeth or for capping teeth · CPC title

• A61L27/56

  Porous materials, {e.g. foams or sponges} · CPC title

• A61K47/64

  Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent (peptidic linkers A61K47/65) · CPC title