KDR and VEGF/KDR binding peptides and their use in diagnosis and therapy

Patent metadata

Field	Value
Publication number	US-9446155-B2
Application number	US-201314109508-A
Country	US
Kind code	B2
Filing date	Dec 17, 2013
Priority date	Mar 1, 2002
Publication date	Sep 20, 2016
Grant date	Sep 20, 2016

Abstract

The present invention provides polypeptides, peptide dimer, and multimeric complexes comprising at least one binding moiety for KDR or VEGF/KDR complex, which have a variety of uses wherever treating, detecting, isolating or localizing angiogenesis is advantageous. Particularly disclosed are synthetic, isolated polypeptides capable of binding KDR or VEGF/KDR complex with high affinity (e.g., having a K D <1 μM), and dimer and multimeric constructs comprising these polypeptides.

First claim

What is claimed is:

1. A diagnostic imaging agent comprising a detectable label conjugated to at least one polypeptide having the ability to bind to KDR or VEGF/KDR complex, optionally further comprising a linker or spacer between the polypeptide and the detectable label, wherein the polypeptide comprises an amino acid sequence selected from the group consisting of:

(SEQ ID NO: 263)

AGDSWCSTEYTYCEMIGTGGGK;

(SEQ ID NO: 264)

AGPKWCEEDWYYCMITGTGGGK;

(SEQ ID NO: 265)

AGVWECAKTFPFCHWFGTGGGK;

(SEQ ID NO: 266)

AGWVECWWKSGQCYEFGTGGGK;

(SEQ ID NO: 268)

AGWIQCNSITGHCTSGGTGGGK;

(SEQ ID NO: 269)

AGWIECYHPDGICYHFGTGGGK;

(SEQ ID NO: 270)

AGSDWCRVDWYYCWLMGTGGGK;

(SEQ ID NO: 271)

AGANWCEEDWYYCFITGTGGGK;

(SEQ ID NO: 272)

AGANWCEEDWYYCWITGTGGGK;

(SEQ ID NO: 273)

AGPDWCEEDWYYCWITGTGGGK;

(SEQ ID NO: 274)

AGSNWCEEDWYYCYITGTGGGK;

(SEQ ID NO: 275)

AGPDWCAADWYYCYITGTGGGK;

(SEQ ID NO: 276)

AGPEWCEVDWYYCWLLGTGGGK;

(SEQ ID NO: 277)

AGPTWCEDDWYYCWLFGTGGGK;

(SEQ ID NO: 278)

AGSKWCEQDWYYCWLLGTGGGK;

(SEQ ID NO: 279)

AGRNWCEEDWYYCFITGTGGGK;

(SEQ ID NO: 280)

AGVNWCEEDWYYCWITGTGGGK;

(SEQ ID NO: 281)

AGANWCEEDWYYCYITGTGGGK;

(SEQ ID NO: 282)

AGQAWVECYAETGYCWPRSWGTGGGK;

(SEQ ID NO: 283)

AGQAWIECYAEDGYCWPRSWGTGGGK;

(SEQ ID NO: 284)

AGVGWVECYQSTGFCYHSRDGTGGGK;

(SEQ ID NO: 285)

AGFTWVECHQATGRCVEWTTGTGGGK;

(SEQ ID NO: 286)

AGDWWVECRVGTGLCYRYDTGTGGGK;

(SEQ ID NO: 287)

AGDSWVECDAQTGFCYSFLYGTGGGK;

(SEQ ID NO: 289)

AGERWVECRAETGCYTWVSGTGGGK;

(SEQ ID NO: 290)

AGGGWVECRAETGHCQEYRLGTGGGK;

(SEQ ID NO: 291)

AGVAWVECYQTTGKCYTFRGGTGGGK;

(SEQ ID NO: 292)

AGEGWVECFANTGACFTYPRGTGGGK;

(SEQ ID NO: 293)

GDYPWCHELSDSVTRFCVPWDPGGGK;

(SEQ ID NO: 294)

GDSRVCWEDSWGGEVCFRYDPGGGK;

(SEQ ID NO: 295)

GDDHMCRSPDYQDHVCMYWDPGGGK;

(SEQ ID NO: 296)

GDPPLCYFVGTQEWHHCNPFDPGGGK;

(SEQ ID NO: 297)

GDDSYCMMNEKGWWNCYLYDPGGGK;

(SEQ ID NO: 298)

G

Assignees

• Dyax Corp
• Bracco Suisse Sa

Inventors

• Sato Aaron K
• Sexton Daniel J
• Dransfield Daniel T
• Ladner Robert Charles
• Arbogast Christophe
• Bussat Philippe
• Fan Hong
• Khurana Sudha
• Linder Karen E
• Marinelli Edmund R
• Nanjappan Palaniappa
• Nunn Adrian D
• Pillai Radhakrishna K
• Pochon Sibylle
• Ramalingam Kondareddiar
• Shrivastava Ajay
• Song Bo
• Swenson Rolf E
• Von Wronski Mathew A
• Yan Feng

Classifications

• A61K49/223 Primary

  Microbubbles, hollow microspheres, free gas bubbles, gas microspheres · CPC title

• C07K14/52

  Cytokines; Lymphokines; Interferons · CPC title

• A61K47/48238

  Human Necessities · mapped topic

• C07K7/08

  having 12 to 20 amino acids (gastrins C07K14/595; somatostatins C07K14/655; melanotropins C07K14/68) · CPC title

• C07K2319/00

  Fusion polypeptide · CPC title