Compositions and methods for sample preparation

Patent metadata

Field	Value
Publication number	US-9441265-B2
Application number	US-201214369627-A
Country	US
Kind code	B2
Filing date	Dec 28, 2012
Priority date	Dec 29, 2011
Publication date	Sep 13, 2016
Grant date	Sep 13, 2016

Abstract

The invention relates generally to compositions and methods for disrupting cells (e.g., disrupting cellular and nuclear membranes). In particular, the invention provides modified solid surfaces (e.g., bead surfaces) and their use in disruption of cellular membranes (e.g., during cellular lysis procedures (e.g., for recovery of nucleic acid (e.g., DNA or RNA) from mechanical cell lysis)). Compositions and methods of the invention find use in a wide range of applications including molecular biology and medical science.

First claim

What is claimed is:

1. A method of isolating a nucleic acid from a sample comprising: a) contacting the sample with a composition comprising a fluorinated organic group modified surface wherein said composition comprising said fluorinated organic group modified surface has less affinity for said nucleic acid than an identical composition without said fluorinated organic group modified surface; b) mechanically disrupting the sample; and c) isolating the nucleic acid from the disrupted sample.

2. The method of claim 1 , wherein the composition comprising the modified surface is a silica bead.

3. The method of claim 2 , wherein the bead is between 90 μm and 500 μm in size.

4. The method of claim 1 , wherein the fluorinated organic group is a fluorinated silane.

5. The method of claim 4 , wherein the fluorinated silane is a perfluorooctyltriethoxysilane.

6. The method of claim 1 , wherein the nucleic acid is a DNA.

7. The method of claim 1 , wherein the sample is a mammalian or a microbial cell.

8. The method of claim 1 , wherein said composition comprising a fluorinated organic group modified surface is a bead surface.

9. The method of claim 8 , wherein said mechanically disrupting the sample is bead beating.

10. The method of claim 9 , wherein said composition comprises a dispersing agent.

11. The method of claim 10 , wherein said dispersing agent is toluene.

12. The method of claim 9 , wherein said composition comprises a catalyst or reaction promoter.

13. The method of claim 12 , wherein said reaction promoter is a base.

Assignees

• Ibis Biosciences Inc

Inventors

• Chiesl Thomas N

Classifications

• C12N15/1006 Primary

  by means of a solid support carrier, e.g. particles, polymers · CPC title

• C12Q1/6806 Primary

  Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence) · CPC title