Formation of immobilized biological layers for sensing

Patent metadata

Field	Value
Publication number	US-9441259-B2
Application number	US-201414549927-A
Country	US
Kind code	B2
Filing date	Nov 21, 2014
Priority date	Dec 20, 2007
Publication date	Sep 13, 2016
Grant date	Sep 13, 2016

Abstract

The invention is directed to enzyme immobilization compositions comprising: one or more enzymes, a humectant, an acrylic-based monomer, a water-soluble organic photo-initiator and a water-soluble acrylic-based cross-linker in a substantially homogeneous aqueous mixture. The invention is also directed to methods for forming sensors comprising such compositions and to apparati for forming arrays of immobilized layers on an array of sensors by dispensing such compositions onto a substrate.

First claim

What is claimed is:

1. An apparatus for forming an immobilized layer on a substantially planar surface, the apparatus comprising:
a dispensing head configured to dispense a controlled volume of a photoformable matrix at a pre-selected location on said surface, wherein the photoformable matrix comprises a biologically active material selected from the group consisting of protein, enzyme, antibody, antibody fragment, RNA, single stranded DNA and double stranded DNA, a humectant, an acrylic-based monomer, a water soluble organic photo-initiator, and a water soluble acrylic-based cross-linker in a homogenous aqueous mixture;
a UV radiation source configured to generate a beam of radiation; and
a computing device comprising a computer program configured to control the dispensing head, the UV radiation source, and registration and alignment of the dispensing head and the UV radiation source such that the beam of radiation is focused onto an area substantially covering said pre-selected location at a predetermined time and for a predetermined duration, at a predetermined intensity, after said matrix has been dispensed by said dispensing head.

2. The apparatus of claim 1 , wherein said substantially planar surface is selected from the group consisting of silicon wafer, alumina wafer, liquid crystal substrate, glass substrate and plastic substrate and flexible plastic substrate.

3. The apparatus of claim 1 , wherein said dispensing head comprises a syringe needle with a reservoir for said matrix, and a pneumatic pump and valve configured to dispense the controlled volume from said syringe onto said surface.

4. The apparatus of claim 1 , wherein the controlled volume is in the range of about 5 nL to about 1 μL.

5. The apparatus of claim 1 , wherein said pre-selected location has an area in the range of about 10 square microns to about 75 square millimeters.

6. The apparatus of claim 1 , wherein said pre-selected location is substantially circular with radial dimensions in the range of about 5 μm to about 5 mm.

7. The apparatus of claim 1 , wherein said UV radiation source is a mercury lamp.

8. The apparatus of claim 1 , wherein the computing program is further configured to control the registration and alignment of the UV radiation source such that the beam of radiation is focused on a selected area of said surface and illuminates an area in the range of about 10 square microns to about 75 square millimeters.

9. The apparatus of claim 1 , further comprising an X-Y tray and vacuum chuck configured to change a relative position of said surface with respect to said dispensing head and said UV radiation source for forming an array of immobilized enzyme layers at a set of pre-selected locations.

10. An apparatus for forming an array of immobilized enzyme layers on an array of sensors on a substantially planar surface comprising:
a dispensing head configured to dispense controlled volumes of a photoformable enzyme-containing matrix at a pre-selected set of locations on said surface;
a UV radiation source configured to generate a beam of radiation; and
a computing device comprising a computer program configured to control the dispensing head, the UV radiation source, and registration and alignment of the dispensing head and the UV radiation source such that the beam of radiation is focused onto an area substantially covering an Nth minus X of the pre-selected locations while dispensing occurs at the Nth of the pre-selected locations, where X is equal to an integer from 1 to 10, and radiation is applied to the Nth minus X of the pre-selected locations for a predetermined duration.

Assignees

• Abbott Point Of Care Inc

Inventors

• Collier Gordon Bruce
• Macleod Jason Andrew
• Wong Anjulia
• Nemeth Attila Csaba

Classifications

• B05C5/0291

  the material being discharged on the work through discrete orifices as discrete droplets, beads or strips that coalesce on the work or are spread on the work so as to form a continuous coating · CPC title

• B05C9/12

  the auxiliary operation being performed after the application (B05C9/14 takes precedence) · CPC title

• C12Q1/58

  involving urea or urease · CPC title

• B05C11/1034

  specially designed for conducting intermittent application of small quantities, e.g. drops, of coating material (B05C5/0225, B05C11/1026 take precedence) · CPC title

• C12Q1/006

  for glucose · CPC title