Sorbent for endotoxins

Patent metadata

Field	Value
Publication number	US-9440019-B2
Application number	US-201113806184-A
Country	US
Kind code	B2
Filing date	Jun 22, 2011
Priority date	Jun 24, 2010
Publication date	Sep 13, 2016
Grant date	Sep 13, 2016

Abstract

The invention relates to a sorption agent for removing endotoxins from a biological fluid, comprising a water-insoluble, porous carrier having a neutral hydrophobic surface wherein the surface of the carrier has an adsorptive coating made of polymyxin and albumin, wherein polymyxin and albumin are bonded to the surface of the carrier in a noncovalent manner. The invention further relates to a method for producing such a sorption agent. The sorption agent is used in extracorporeal blood purification, in particular in order to treat individuals, who have sepsis.

First claim

The invention claimed is:

1. A sorption agent for removing endotoxins from blood or blood plasma, comprising:
a water-insoluble, porous carrier having a neutral hydrophobic surface wherein the surface of the carrier has an adsorptive coating made of polymyxin B and human serum albumin, wherein the polymyxin B and human serum albumin are noncovalently attached to the surface of the carrier by a hydrophobic interaction.

2. The sorption agent according to claim 1 , wherein the carrier is a hydrophobic polymer.

3. The sorption agent according to claim 2 , wherein the hydrophobic polymer is a cross-linked polystyrene polymer.

4. The sorption agent according to claim 1 , wherein the carrier has a mean pore size of at least 15 nm.

5. The sorption agent according to claim 1 , wherein the carrier has a mean pore size of not greater than 120 nm.

6. The sorption agent according to claim 1 , wherein the sorption agent has the form of microparticles.

7. The sorption agent according to claim 6 , wherein the microparticles have a particle size of 20 μm or less.

8. A sorption apparatus, containing the sorption agent according to claim 1 .

9. A plasma circuit, containing a suspension of the sorption agent according to claim 1 .

10. A method of producing a sorption agent according to claim 1 , comprising the steps of:
a) providing a water-insoluble, porous carrier which has a neutral, hydrophobic surface,
b) adsorptively coating the surface of the carrier with polymyxin B by contacting and incubating the carrier with an aqueous, polymyxin B-containing solution,
c) washing the carrier to remove any unbound polymyxin B,
d) adsorptively coating the polymyxin B-coated surface of the carrier with human serum albumin by contacting and incubating the carrier with an aqueous, human serum albumin-containing solution, and
e) as appropriate, washing the carrier to remove any unbound human serum albumin.

11. The method according to claim 10 , wherein the carrier is autoclaved between the steps b) and c) or between the steps c) and d).

12. A method for removing endotoxins from blood or blood plasma, comprising:
bringing blood or blood plasma contaminated with endotoxins into contact with the sorption agent according to claim 1 .

13. The method according to claim 12 , carried out for the treatment of a sepsis.

14. The method according to claim 12 , wherein the sorption agent is included in a Microspheres-based Detoxification System (MDS).

15. The sorption agent according to claim 3 , wherein the hydrophobic polymer is a polystyrene-divinyl benzene copolymer.

16. The sorption agent according to claim 4 , wherein the carrier has a mean pore size of at least 30 nm.

17. The sorption agent according to claim 7 , wherein the microparticles have a particle size of 8 μm or less.

18. The sorption agent according to claim 7 , wherein the microparticles have a particle size of 5 μm or less.

Assignees

• Falkenhagen Dieter
• Harm Stephan
• Hartmann Jens
• Weber Viktoria
• Fresenius Medical Care Deutschland Gmbh

Inventors

• Falkenhagen Dieter
• Harm Stephan
• Hartmann Jens
• Weber Viktoria

Classifications

• B01J20/3274

  Proteins, nucleic acids, polysaccharides, antibodies or antigens · CPC title

• B01J20/321

  consisting of a polymer obtained by reactions involving only carbon to carbon unsaturated bonds · CPC title

• B01J20/267

  Cross-linked polymers · CPC title

• B01J20/3255

  comprising a cyclic structure containing at least one of the heteroatoms nitrogen, oxygen or sulfur, e.g. heterocyclic or heteroaromatic structures · CPC title

• B01J20/3248

  the functional group or the linking, spacer or anchoring group as a whole comprising at least one type of heteroatom selected from a nitrogen, oxygen or sulfur, these atoms not being part of the carrier as such · CPC title