Method for observing stem cells, method for removal of cell region in state tending toward differentiation, and device for observing stem cells

Patent metadata

Field	Value
Publication number	US-9435734-B2
Application number	US-201514806999-A
Country	US
Kind code	B2
Filing date	Jul 23, 2015
Priority date	Nov 8, 2011
Publication date	Sep 6, 2016
Grant date	Sep 6, 2016

Abstract

A method for observing stem cells by an observation device 1 comprises, placing stem cells C in a petri dish 11 , mounting the petri dish 11 on a waveguide 21 via water 13 , emitting illumination light L 1 into the waveguide 21 and emitting the illumination light L 1 to the stem cells C in the petri dish 11 via the water 13 , and detecting scattered light L 2 , the scattered light L 2 being the illumination light L 1 emitted to the stem cells C that is scattered by the stem cells C and has passed through the waveguide 21 . Then, in the light image detected by means of the scattered light L 2 , a region that is markedly darker than other regions is identified as being in the state tending toward differentiation.

First claim

The invention claimed is:

1. A method for observing stem cells, comprising:
irradiating stem cells placed on a transparent member with oblique illumination light;
capturing a scattered light image of the stem cells by a camera having a two dimensional pixel structure, in which a plurality of pixels are two-dimensionally arranged; and
identifying a stem cell that is in the state tending toward differentiation in the sterm cells based on the scattered light image,
wherein the irradiating irradiates the stem cells with the oblique illumination light via the transparent member.

2. The method according to claim 1 , wherein the identifying identifies a darker portion in the scattered light image of the stem cell that is in the state tending toward differentiation.

3. The method according to claim 1 , wherein the capturing images light that is scattered by the stem cells and passed through the transparent member.

4. The method according to claim 1 , further comprising,
irradiating the stem cells placed on the transparent member with transmitted illumination light; and
capturing a transmitted light image of the stem cells by the camera.

5. The method according to claim 1 , further comprising,
removing the identified stem cell from the stem cells.

6. The method according to claim 1 , wherein the stem cells are iPS cells.

7. An apparatus for observing stem cells, comprising:
a light source configured to output light;
an illumination optics configured to input the output light and irradiate stem cells placed on a transparent member with oblique illumination light;
a camera having a two dimensional pixel structure, in which a plurality of pixels are two-dimensionally arranged and configured to capture a scattered light image of the stem cells; and
a display configured to display the scattered light image, thereby identifying a stem cell that is in the state tending toward differentiation in the stem cells,
wherein the illumination optics irradiates the stem cells with the oblique illumination light via the transparent member.

8. The apparatus according to claim 7 , wherein the camera images light that is scattered by the stem cells and passed through the transparent member.

9. The apparatus according to claim 7 , wherein the illumination optics comprises a light guide having a surface on which the transparent member can be mounted and another surface inputting the output light.

10. The apparatus according to claim 9 , wherein the camera images light that is scattered by the stem cells and passed through the transparent member and the light guide.

11. The apparatus according to claim 7 , wherein the stem cells are iPS cells.

12. An apparatus for observing stem cells, comprising:
a light source configured to output light;
an illumination optics configured to input the output light and irradiate stem cells placed on a transparent member with oblique illumination light;
a camera having a two dimensional pixel structure, in which a plurality of pixels are two-dimensionally arranged and configured to capture a scattered light image of the stem cells; and
a display configured to display the scattered light image, thereby identifying a stem cell that is in the state tending toward differentiation in the stem cells,
wherein the illumination optics comprises a light guide having a surface on which the transparent member can be mounted and another surface inputting the output light.

13. The apparatus according to claim 12 , wherein the camera images light that is scattered by the stem cells and passed through the transparent member.

14. The apparatus according to claim 12 , wherein the stem cells are iPS cells.

Assignees

• Hamamatsu Photonics Kk

Inventors

• Sugiyama Norikazu
• Kataoka Takuji
• Arata Ikuo

Classifications

• G02B21/36

  arranged for photographic purposes or projection purposes (G02B21/18 takes precedence){or digital imaging or video purposes including associated control and data processing arrangements (image data processing per se G06T)} · CPC title

• G01N33/5005

  involving human or animal cells (immunoassay G01N33/56966; immunoassays of protozoa G01N33/56905; protozoa in screening assays C12Q1/025) · CPC title

• G01N2201/0446

  Multicell plate, sequential · CPC title

• G01N33/4833

  of solid biological material, e.g. tissue samples, cell cultures (tissue in vivo A61B5/00; cell suspensions G01N33/48735) · CPC title

• G01N21/59

  Transmissivity (G01N21/25 takes precedence) · CPC title