Transgenic plants with enhanced growth characteristics

Patent metadata

Field	Value
Publication number	US-9434956-B2
Application number	US-201213714948-A
Country	US
Kind code	B2
Filing date	Dec 14, 2012
Priority date	Aug 29, 2008
Publication date	Sep 6, 2016
Grant date	Sep 6, 2016

Abstract

The invention relates to transgenic plants exhibiting dramatically enhanced growth rates, greater seed and fruit/pod yields, earlier and more productive flowering, more efficient nitrogen utilization, increased tolerance to high salt conditions, and increased biomass yields. In one embodiment, transgenic plants engineered to over-express both glutamine phenylpyruvate transaminase (GPT) and glutamine synthetase (GS) are provided. The GPT+GS double-transgenic plants of the invention consistently exhibit enhanced growth characteristics, with T0 generation lines showing an increase in biomass over wild type counterparts of between 50% and 300%. Generations that result from sexual crosses and/or selfing typically perform even better, with some of the double-transgenic plants achieving an astounding four-fold biomass increase over wild type plants.

First claim

What is claimed is:

1. A method for producing a transgenic plant having increased biomass yield relative to an analogous wild type or untransformed plant, comprising:
(a) introducing a glutamine phenylpyruvate transaminase (GPT) transgene into a plurality of plants, wherein the GPT transgene encodes a GPT polypeptide having (1) an amino acid sequence that has at least 85% sequence identity to SEQ ID NO: 2 and (2) GPT catalytic activity;
(b) introducing a glutamine synthetase (GS) transgene into the plurality of plants or progeny of the plurality of plants, wherein said GS transgene encodes a GS polypeptide having (1) an amino acid sequence that has at least 85% sequence identity to SEQ ID NO: 7 and (2) GS catalytic activity;
(c) expressing the GPT transgene and the GS transgene in the plurality of plants or the progeny thereof; and,
(d) selecting from said plurality of plants or the progeny thereof a transgenic plant having an increased biomass yield relative to an analogous wild type or untransformed plant of the same species.

2. The method according to claim 1 , wherein the plant further has one or more characteristics selected from the group consisting of earlier flowering, earlier budding, increased plant height, increased flowering, increased budding, larger leaves, increased fruit or pod yield and increased seed yield.

3. The method according to claim 1 , wherein the GS transgene is a GS 1 transgene.

4. The method according to claim 1 , wherein each transgene is linked to a plant promoter that is heterologous to the plant.

5. The method according to claim 1 , wherein the GPT transgene encodes a polypeptide having an amino acid sequence selected from the group consisting of:
(a) SEQ ID NO: 2 and
(b) an amino acid sequence that has at least 90% sequence identity to SEQ ID NO: 2 and has GPT activity.

6. The method according to claim 1 , wherein the GS transgene encodes a polypeptide having an amino acid sequence selected from the group consisting of:
(a) SEQ ID NO: 7 and
(b) an amino acid sequence that has at least 90% sequence identity to SEQ ID NO: 7 and has GS activity.

7. The method according to claim 1 , wherein the GPT and GS transgenes are incorporated into the genome of the selected transgenic plant.

8. The method according to claim 1 , wherein the plant is a monocotyledonous plant.

9. The method according to claim 1 , wherein the plant is a dicotyledonous plant.

10. The method according to claim 1 , wherein said transgenic plant further has one or more characteristics selected from the group consisting of an enhanced growth rate, increased seed yield, increased flower or flower bud yield, increased fruit or pod yield, larger leaves, increased GPT activity, increased GS activity, increased 2-oxoglutaramate levels and increased nitrogen use efficiency, when compared to an analogous wild-type or untransformed plant.

11. The method according to claim 1 , wherein the transgenic plant is selected from the group consisting of plants of the families Poaceae, Gossypium, Fabaceae, Rutaceae, Rubiaceae, Cucurbitaceae, Rosaceae, Asteraceae, Amaranthaceae or Brassicaceae.

12. The method according to claim 1 , wherein the transgenic plant is selected from the group consisting of plants of the genera Avena, Hordeum, Oryza, Panicum, Phleum, Saccharum, Secale, Sorghum, Triticum, Zea, Pennisetum, Lycopersicon, Capsicum, Fagopyrum, Triticosecale, Chenopodium, Digitaria, Manihot, Ipomoea, Olea, Daucus, Pastinaca, Raphanus, Dioscorea, Armoracia, Elaeis, Linum, Carthamus, Sesamum, Vitis , or Solanum.

13. The method according to claim 1 , wherein said transgenic plant produces more 2-oxo-glutaramate relative to an analogous wild type or untransformed plant of the same species.

14. The method according to claim 1 , wherein the transgenic plant has an increased leaf-to-root ratio of GS activity in comparison to an analogous wild type or untransformed plant of the same species.

15. The method according to claim 1 , wherein the transgenic plant has an increased leaf-to-root ratio of 2-oxo-glutaramate in comparison to an analogous wild type or untransformed plant of the same species.

16. The method according to claim 1 , wherein the GS transgene encodes a polypeptide having the amino acid sequence according to SEQ ID NO: 7.

17. The method according to claim 1 , wherein the GPT transgene encodes a polypeptide having the amino acid sequence according to SEQ ID NO: 2.

18. A method for generating and selecting transgenic plants having increased production of 2-oxo-glutaramate relative to an analogous wild type or untransformed plant, comprising:
(a) introducing a glutamine phenylpyruvate transaminase (GPT) transgene into a plurality of plant cells, wherein the GPT transgene encodes a GPT polypeptide having (1) an amino acid sequence that has at least 85% sequence identity to SEQ ID NO: 2 and (2) GPT catalytic activity,
(b) introducing a glutamine synthetase (GS) transgene into said plurality of plant cells wherein said GS transgene encodes a GS polypeptide having (1) an amino acid sequence that has at least 85% sequence identity to SEQ ID NO: 7 and (2) GS catalytic activity;
(c) producing a plurality of transgenic plants from the plurality of plant cells; and
(d) selecting from said plurality of plants a transgenic plant that produces more 2-oxo-glutaramate relative to an analogous wild type or untransformed plant.

19. The method according to claim 18 , wherein the step of introducing said GPT transgene into said plurality of plant cells comprises inoculation of said plurality of plants cells with Agrobacterium transformed with said GPT transgene.

20. The method according to claim 19 , further comprising the step of using a selectable marker to identify Agrobacterium transformed with said GPT transgene prior to inoculation of said plurality of plant cells.

21. The method according to claim 18 , wherein the step of introducing said GS transgene into said plurality of plant cells comprises inoculation of the plurality of plants cells with Agrobacterium transformed with said GS transgene.

22. The method according to claim 21 , further comprising the step of using a selectable marker to identify Agrobacterium transformed with said GS transgene prior to inoculation of said plurality of plant cells.

23. The method according to claim 18 , wherein the plant has at least one enhanced growth characteristic selected from the group consisting of increased biomass yield, increased nitrogen use efficiency, earlier flowering, earlier budding, increased plant height, increased flowering, increased budding, larger leaves, increased fruit or pod yield and increased seed yield.

24. The method according to claim 18 , wherein each transgene is linked to a plant promoter that is heterologous to the plant.

25. The method according to claim 18 , wherein the GPT transgene encodes a polypeptide having an amino acid sequence selected from the group consisting of:
(a) SEQ ID NO:2 and
(b) an amino acid sequence that has at least 90% sequence identity to and has GPT catalytic activity.

26. The method according to claim 18 , wherein the GS transgene encodes a polypeptide having an amino acid sequence selected form the group consisting of (a) and (b) an amino acid sequence that has at least 90% sequence identity to SEQ ID NO: 7 and has GS catalytic activity.

27. The method according to claim 18 , wherein th

Assignees

• Los Alamos Nat Security Llc
• Univ Maine System

Inventors

• Unkefer Pat J
• Anderson Penelope S
• Knight Thomas J

Classifications

• C12N9/1096

  transferring nitrogenous groups (2.6) · CPC title

• C12N15/8261 Primary

  with agronomic (input) traits, e.g. crop yield · CPC title

• Y02A40/146

  Genetically Modified [GMO] plants, e.g. transgenic plants · CPC title

• C12N9/93

  Ligases (6) · CPC title

• C12Y206/01064

  Glutamine-phenylpyruvate transaminase (2.6.1.64) · CPC title