Small interfering RNAS with target-specific seed sequences

Patent metadata

Field	Value
Publication number	US-9434942-B2
Application number	US-201213980714-A
Country	US
Kind code	B2
Filing date	Feb 14, 2012
Priority date	Feb 14, 2011
Publication date	Sep 6, 2016
Grant date	Sep 6, 2016

Abstract

Disclosed are methods for design and synthesis of siRNA libraries, siRNA libraries produced thereby, siRNA molecules, and uses thereof.

First claim

What is claimed is:

1. A siRNA molecule comprising the nucleotide sequence of any one of SEQ ID NOs: 1-14 and wherein the siRNA molecule reduces the number of soybean cyst nematode cysts on soybean roots.

2. An artificial RNA molecule comprising the siRNA molecule of claim 1 , wherein the artificial RNA molecule is encoded by the nucleotide sequence of any one of SEQ ID NOs: 16-29.

3. A vector comprising the siRNA molecule of claim 1 , wherein the vector is encoded by the nucleotide sequence of any one of SEQ ID NOs: 31-44.

Assignees

• Huang Xiang
• Mcneill Thomas
• Schweiner Mike
• Syngenta Participations Ag

Inventors

• Huang Xiang
• Mcneill Thomas
• Schweiner Mike

Classifications

• C12Q1/6888 Primary

  for detection or identification of organisms · CPC title

• C12N15/113 Primary

  Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; {Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing (when used in plants C12N15/8218)} · CPC title

• C12N2330/31

  Libraries, arrays · CPC title

• C12Q2600/13

  Plant traits · CPC title

• C12N2310/113

  targeting other non-coding nucleic acids, e.g. antagomirs · CPC title