Instrument for cassette for sample preparation

Patent metadata

Field	Value
Publication number	US-9434939-B2
Application number	US-201615006494-A
Country	US
Kind code	B2
Filing date	Jan 26, 2016
Priority date	Dec 27, 2006
Publication date	Sep 6, 2016
Grant date	Sep 6, 2016

Abstract

A parallel processing system for processing samples is described. In one embodiment, the parallel processing system includes an instrument interface parallel controller to control a tray motor driving system, a close-loop heater control and detection system, a magnetic particle transfer system, a reagent release system, a reagent pre-mix pumping system and a wash buffer pumping system.

First claim

The invention claimed is:

1. A system comprising:
a network; and
a plurality of instruments, wherein:
each instrument in the plurality of instruments is configured to receive a plurality of cassettes;
each instrument in the plurality of instruments comprises a particle transfer system including a plurality of valve shafts and a first actuator assembly;
each instrument in the plurality of instruments comprises a computer; and
each instrument in the plurality of instruments comprises a communication device, wherein the communication device is configured to couple the instrument to the network;
wherein the first actuator assembly is configured to move the plurality of valve shafts between a first position and a second position; and
the first actuator assembly is configured to rotate each valve shaft from the plurality of valve shafts when the plurality of valve shafts is in the second position.

2. The system of claim 1 wherein each cassette of the plurality of cassettes comprises a valve member defining a cavity within which a particle can be disposed, the valve member configured to transfer the particle between a first chamber of the cassette and a second chamber of the cassette.

3. The system of claim 2 wherein the first actuator assembly is configured to apply a magnetic force produced by a magnetic portion of each valve shaft to attract the particle into the cavity of the valve member of the corresponding cassette from the plurality of cassettes.

4. The system of claim 3 wherein the first actuator assembly is configured to rotate when the particle is transferred between the first chamber of the cassette and the second chamber of the cassette.

5. The system of claim 2 wherein each instrument in the plurality of instruments comprises a reagent release system comprising a plurality of protrusions and a second actuator assembly.

6. The system of claim 5 wherein each cassette comprises a first reagent chamber and a second reagent chamber, and wherein the second actuator assembly is configured to move the plurality of protrusions between a first protrusion position, in which each protrusion from the plurality of protrusions is substantially aligned with the first reagent chamber from the corresponding cassette and a second protrusion position, in which each protrusion from the plurality of protrusions is substantially aligned with the second reagent chamber from the corresponding cassette.

7. The system of claim 6 wherein the second actuator assembly is configured to move a protrusion from the plurality of protrusions into contact with a plunger associated with the first chamber of the corresponding cassette to transfer a first reagent from the first reagent chamber into the first chamber, the second actuator assembly configured to move the protrusion from the plurality of protrusions into contact with a plunger associated with the second reagent chamber of the corresponding cassette to transfer a second reagent from the second reagent chamber into the first chamber.

8. The system of claim 7 wherein the second actuator assembly is configured to move the plurality of protrusions along a first axis between the first protrusion position and the second protrusion position, the second actuator assembly is further configured to move the protrusion from the plurality of protrusions along a second axis into contact with the plunger associated with the first reagent chamber.

9. The system of claim 7 wherein the second actuator assembly is configured to move at least a portion of the protrusion from the plurality of protrusions into the first reagent chamber to transfer the first reagent from the first reagent chamber into the first chamber.

10. The system of claim 7 wherein each cassette from the plurality of cassettes includes a puncturable member disposed between the first chamber and the first reagent chamber; and the second actuator assembly is configured to move the protrusion from the plurality of protrusions such that the plunger associated with the first reagent chamber punctures a portion of the puncturable member.

11. The system of claim 7 wherein each cassette from the plurality of cassettes includes a pump configured to mix at least one of the first reagent or the second reagent with the particle within the first chamber; and the second actuator assembly is configured to actuate each pump from the plurality of cassettes.

12. The system of claim 1 wherein the communication device comprises a universal asynchronous receiver transmitter (UART).

13. The system of claim 12 wherein the communication device comprises a universal serial bus (USB).

14. The system of claim 13 wherein the communication device comprises a controller area network bus (CANBUS).

15. The system of claim 1 wherein the network is a wireless network.

16. The system of claim 1 wherein the network is a wide area network (WAN).

17. The system of claim 1 wherein the network is a control area network (CAN).

18. The system of claim 1 wherein the network is a token ring.

19. The system of claim 1 wherein the network is coupled to the internet.

20. The system of claim 1 wherein the network is an Ethernet network.

Assignees

• Luminex Corp

Inventors

• Yu Steve Jia Chang
• Ching Jesus
• Lee Phillip You Fai
• Hu David Hsiang

Classifications

• C12N15/1013 Primary

  by using magnetic beads · CPC title

• G01N2035/00881

  network configurations · CPC title

• B01L2400/0478

  pistons · CPC title

• C12Q1/6806 Primary

  Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay (C12Q1/6804 takes precedence) · CPC title

• G01N2035/00574

  Means for distributing beads · CPC title