Plants having stress related traits and methods for making the same

Patent metadata

Field	Value
Publication number	US-9434773-B2
Application number	US-201213617207-A
Country	US
Kind code	B2
Filing date	Sep 14, 2012
Priority date	Sep 14, 2011
Publication date	Sep 6, 2016
Grant date	Sep 6, 2016

Abstract

This invention relates generally to a plant cell with increased yield, preferably under condition of transient and repetitive abiotic stress as compared to a corresponding non-transformed wild type plant cell by increasing or generating one or more activities of RING proteins in plants.

First claim

What is claimed is:

1. A transgenic monocotyledous plant comprising in its genome a stably-integrated, recombinant DNA construct comprising a polynucleotide operably linked to at least one regulatory element, wherein said polynucleotide has a nucleic acid sequence encoding SEQ ID NO: 2, and wherein said monocotyledous plant exhibits increased drought tolerance when compared to a control plant not comprising said recombinant DNA construct.

2. The transgenic monocotyledous plant of claim 1 , wherein the polynucleotide comprises SEQ ID NO: 1 or SEQ ID NO: 3.

3. The transgenic monocotyledous plant of claim 1 , wherein the plant is a maize plant, wheat plant or rice plant.

4. The transgenic monocotyledous plant of claim 2 , wherein the plant is a maize plant, wheat ant or rice plant.

5. The transgenic monocotyledons plant of claim 1 , wherein said plant has an increased yield when grown under drought stress as compared to a non-transformed control plant of the seine species.

6. The transgenic monocotyledous plant of claim 1 , wherein the plant further comprises at least one additional agronomic trait, herbicide trait or insecticide trait.

7. A method of increasing drought and/or heat tolerance in a monocotyledous plant, comprising: (a) introducing into a regenerable plant cell a recombinant DNA construct comprising a polynucleotide operably linked to at least one regulatory sequence, wherein the polynucleotide has a nucleic acid sequence encoding SEQ ID NO: 2; and (b) regenerating a transgenic plant from the regenerable plant cell after step (a), wherein the transgenic plant comprises stably-integrated in its genome the recombinant DNA construct and the transgenic plant exhibits increased drought tolerance when compared to a control plant not comprising the recombinant DNA construct.

8. The method according to claim 7 , further comprising: (c) obtaining a progeny plant derived from the transgenic plant, wherein said progeny plant comprises stably-integrated in its genome the recombinant DNA construct and exhibits increased drought tolerance when compared to a control plant not comprising the recombinant DNA construct.

9. The method according to claim 7 , wherein the polynucleotide comprises SEQ ID NO: 1 or SEQ ID NO: 3.

10. The method according to claim 7 , wherein the plant further comprises at least one additional agronomic trait, herbicide trait or insecticide trait.

11. A method of improving drought tolerance in a corn line comprising crossing a corn plant of said corn line with a drought tolerant transgenic corn plant having a recombinant DNA expressing a protein having the amino acid sequence of SEQ ID NO:2 to produce a corn seed having the recombinant DNA.

12. A method for increasing yield in a corn crop subject to water deficit during its growth, said method comprising planting transgenic seeds having a stably-integrated, recombinant DNA, wherein the stably-integrated, recombinant DNA comprises the nucleic amino acid sequence of SEQ ID NO:1 or SEQ ID NO: 3 or wherein the stably-integrated, recombinant DNA encodes a protein having the amino acid sequence of SEQ ID NO:2 and allowing said seeds to grow to mature corn plants while being subjected to drought during at least one ten day period during vegetative phase of growth.

13. A drought tolerant transgenic corn plant having a recombinant DNA expressing a protein having the amino acid sequence of SEQ ID NO:2.

14. The transgenic corn plant of claim 13 , wherein said corn plant further comprises at least one additional agronomic trait, herbicide trait or insecticide trait.

15. Transgenic seed for growing a drought tolerant corn plant, said seed comprising in its genome a stably-integrated, recombinant DNA encoding a protein having the amino acid sequence of SEQ ID NO: 2.

16. A DNA construct comprising a nucleic acid encoding the polypeptide of SEQ ID NO: 2, wherein the nucleic acid is operably linked to a heterologous regulatory element and wherein SEQ ID NO: 2 increases the maintenance of membrane integrity under abiotic stress in a plant cell.

17. The DNA construct of claim 16 , wherein the nucleic acid has been optimized for expression in a plant.

18. The DNA construct of claim 16 , wherein the nucleic acid comprises the nucleotide sequence of SEQ ID NO: 3.

19. A plant expression vector comprising the DNA construct of claim 16 .

20. A transgenic plant cell comprising, stably-integrated in its genome, the DNA construct of claim 16 .

21. A transgenic plant cell culture, plant tissue, or whole plant comprising the transgenic plant cell of claim 20 .

22. A method for making a transgenic monocotyledous plant having an drought related trait relative to a control plant, comprising introducing a recombinant construct into a plant cell, plant, or part thereof, wherein the drought related trait is one or more of: (i) increased early vigor; (ii) increased biomass; (iii) increased total seed yield per plant; (iv) increased seed filling rate; (v) increased harvest index; (vi) increased thousand kernel weight, (vii) increased abiotic stress resistance, or (viii) increased nutrient uptake efficiency, and wherein the construct comprises (a) a nucleic acid sequence encoding the polypeptide of SEQ ID NO: 2; (b) one or more control sequences capable of driving expression of the nucleic acid sequence of (a) and optionally (c) a transcription termination sequence.

Assignees

• Reddy Avutu S
• Yuejin Sun
• Merlo Donald J
• Ni Weiting W
• Pareddy Dayakar
• Gao Zhifang
• Bradfisch Gregory A
• Dow Agrosciences Llc

Inventors

• Reddy Avutu S
• Yuejin Sun
• Merlo Donald J
• Ni Weiting W
• Pareddy Dayakar
• Gao Zhifang
• Bradfisch Gregory A

Classifications

• C07K14/415 Primary

  from plants · CPC title

• C12N9/93

  Ligases (6) · CPC title

• C12Y603/02019

  Ubiquitin-protein ligase (6.3.2.19), i.e. ubiquitin-conjugating enzyme · CPC title

• C12N15/8273

  for drought, cold, salt resistance · CPC title