Media for membrane ion exchange chromatography based on polymeric primary amines, sorption device containing that media, and chromatography scheme and purification method using the same

Patent metadata

Field	Value
Publication number	US-9433922-B2
Application number	US-22149608-A
Country	US
Kind code	B2
Filing date	Aug 4, 2008
Priority date	Aug 14, 2007
Publication date	Sep 6, 2016
Grant date	Sep 6, 2016

Abstract

Media and devices, such as anion exchangers including such media, wherein the media is a membrane having a surface coated with a polymer such as a polyallylamine. The resulting membrane offers stronger binding of protein impurities and superior removal of host cell proteins from biological samples than conventional ligands based on quaternary ammonium salts, including trimethylammonium ligands. Also described is a chromatography scheme and method for purifying monoclonal antibodies, wherein the anion exchange sorber is placed downstream of an affinity column (such as Protein A or Protein G affinity column) and optionally one or more polishing devices such as cationic exchange columns. Little or no dilution of the cation exchanger pool (or affinity column exchange pool where no cation exchanger is used) is necessary to lower the conductivity of the sample. The sorber functions well to strongly bind host cell proteins and other impurities in biological samples even at high conductivities and pH.

First claim

What is claimed is:

1. A porous sorptive media comprising a substrate having a first external side and a second external side, both sides being porous, and a porous thickness between them, said substrate having a sorptive material substantially covering the solid matrix of the substrate and said first and second external surfaces, said sorptive material comprising protonated polyallylamine crosslinked with polyethylene glycol diglycidyl ether, wherein said crosslinked protonated polyallylamine is present in an amount effective to produce a binding capacity of about 60 g/L BSA binding at 200 mM NaCl, and the thickness of sorptive material substantially covering said first and second external surfaces is 10-50 nm.

2. The porous sorptive media of claim 1 , wherein said substrate comprises a microporous membrane.

3. The porous sorptive media of claim 2 , wherein said membrane comprises a polyolefin.

4. The porous sorptive media of claim 3 , wherein said polyolefin is polyethylene.

5. The porous sorptive media of claim 1 , wherein said substrate is an ultrahigh molecular weight polyethylene membrane.

6. The porous sorptive media of claim 1 , having a nucleic acid binding capacity of greater than 5 mg/ml.

7. The porous sorptive media of claim 6 , wherein said nucleic acid is DNA.

8. The porous sorptive media of claim 1 , wherein said substrate comprises a non-woven material.

9. The porous sorptive media of claim 8 , wherein said non-woven material is a mat or fabric.

Assignees

• Kozlov Mikhail
• Moya Wilson
• Phillips Michael W
• Ramaswamy Senthilkumar
• Gagnon Brian
• Emd Millipore Corp

Inventors

• Kozlov Mikhail
• Moya Wilson
• Phillips Michael W
• Ramaswamy Senthilkumar
• Gagnon Brian

Classifications

• B01D15/3804

  Affinity chromatography · CPC title

• B01D15/362

  Cation-exchange · CPC title

• B01J20/28033 Primary

  Membrane, sheet, cloth, pad, lamellar or mat · CPC title

• B01J47/12

  characterised by the use of ion-exchange material in the form of ribbons, filaments, fibres or sheets, e.g. membranes (electrodialysis or electro-osmosis B01D61/42) · CPC title

• Y10T428/249987

  With nonvoid component of specified composition · CPC title