Detection of a target in a preservative solution

Patent metadata

Field	Value
Publication number	US-9428810-B2
Application number	US-40817909-A
Country	US
Kind code	B2
Filing date	Mar 20, 2009
Priority date	Apr 3, 2000
Publication date	Aug 30, 2016
Grant date	Aug 30, 2016

Abstract

This invention relates to methods, articles and compositions useful in detecting target substances in an alcoholic preservative solution, and for identifying sensors useful for binding to such targets. The methods allow for the simultaneous performance of sufficient fixation of a sample and binding of a detectable sensor to a target of interest in the sample. In one aspect, a method is provided that comprises contacting a sample suspected of containing a target with a detectable sensor molecule known to bind to such target in an alcoholic preservative solution. The method maybe performed in multiplex form to permit simultaneous analysis of a plurality of targets. Methods for identifying a sensor capable of binding to a desired target in an alcoholic preservative solution are also provided. An alcoholic preservative solution comprising one or more such detectable sensors is also provided. Also provided is a sample comprising a bound sensor provided by such a process. Also provided are kits useful for such methods.

First claim

The invention claimed is:

1. A method for the detection of a target in a sample, wherein the target binds to one or more peptide nucleic acid (PNA) probe(s), the method comprising: contacting said sample with one or more PNA probe(s) in an aqueous alcohol solution of at least approximately 20% by volume solution under conditions in which the PNA probe(s) can bind to the target, if present; and detecting the PNA probe(s) bound to the target, wherein the aqueous alcohol solution is at least approximately 40% by volume.

2. The method of claim 1 , wherein the aqueous alcohol solution is at least approximately 50% by volume.

3. The method of claim 1 , wherein the aqueous alcohol solution is greater than approximately 45% by volume and less than approximately 60% by volume.

Assignees

• Linder James
• Cohenford Menashi
• Coffman Erin
• Lentrichia Brian B
• Stender Henrik
• Oliveira Kenneth
• Hologic Inc

Inventors

• Linder James
• Cohenford Menashi
• Coffman Erin
• Lentrichia Brian B
• Stender Henrik
• Oliveira Kenneth

Classifications

• C12Q1/708

  for papilloma · CPC title

• C12Q1/6881 Primary

  for tissue or cell typing, e.g. human leukocyte antigen [HLA] probes · CPC title

• C12Q1/6841

  In situ hybridisation · CPC title

• C12Q1/6888

  for detection or identification of organisms · CPC title

• C12Q2525/107

  incorporating a peptide nucleic acid · CPC title