Methods and compositions for improved cattle longevity and milk production

Patent metadata

Field	Value
Publication number	US-9422608-B2
Application number	US-201314040757-A
Country	US
Kind code	B2
Filing date	Sep 30, 2013
Priority date	Nov 7, 2007
Publication date	Aug 23, 2016
Grant date	Aug 23, 2016

Abstract

A single nucleotide polymorphic site at position 10793 of the bovine POU1F1 gene is associated with improved longevity and milk product traits. Disclosed are nucleic acid molecules, kits, methods of genotyping and marker assisted bovine breeding methods.

First claim

What is claimed is:

1. A bovine breeding method, comprising
1) identifying a bovine animal which comprises adenine at a position of the POU1F1 gene corresponding to position 10793 of SEQ ID NO: 1, by obtaining a nucleic acid sample from the bovine animal and detecting an adenine nucleotide at the position, and
2) using the identified bovine animal as a breeding parent in a breeding process.

2. The method of claim 1 , wherein a bovine animal which comprises homozygously adenine at the position of the POU1F1 gene corresponding to position 10793 of SEQ ID NO: 1 is identified and used as a breeding parent.

3. A method for implanting an embryo comprising: in vitro fertilizing cattle eggs to obtain fertilized eggs, culturing said fertilized eggs into developing embryos, detecting the identity of a nucleotide of a POU1F1 gene of an embryo at a position corresponding to position 10793 of SEQ ID NO: 1, wherein the POU1F1 gene comprises the nucleotide sequence of SEQ ID NO: 1, identifying an embryo that has an adenine at said position, and implanting into a suitable female bovine said identified embryo.

4. The method of claim 3 , wherein the identity of the nucleotide of both copies of the POU1F1 gene in a cell is determined.

5. The method of claim 3 , wherein the nucleotide is detected by sequencing the POU1F1 gene or a relevant fragment thereof.

6. The method of claim 5 , wherein the gene or relevant fragment thereof is isolated from a nucleic acid sample of the embryo via amplification by a polymerase chain reaction of nucleic acid of the embryo.

7. A method for selecting a bull bovine animal as a breeder, the method comprising: obtaining a sample of the bull bovine animal's nucleic acid, wherein the nucleic acid comprises at least a partial POU1F1 gene comprising SEQ ID NO: 1, detecting an adenine nucleotide at a position of the POU1F1 gene of the bull bovine animal corresponding to position 10793 of SEQ ID NO:1, selecting the bull bovine animal that has an adenine at said position, using a bovine cell or tissue from the identified bull bovine animal in a breeding procedure.

8. The method of claim 7 , wherein the bovine cell is a sperm.

9. The method of claim 7 , wherein the nucleotide is detected by sequencing the POU1F1 gene or a relevant fragment thereof.

10. The method of claim 9 , wherein the gene or relevant fragment thereof is isolated from the animal's nucleic acid sample via amplification by a polymerase chain reaction of nucleic acid of the sample.

11. The method of claim 7 , wherein the identity of both copies of the nucleotide of the POU1F1 gene in a cell is determined.

Assignees

• Wisconsin Alumni Res Found

Inventors

• Khatib Hasan
• Huang Wen

Classifications

• C12Q2600/156

  Polymorphic or mutational markers · CPC title

• C12Q1/6876 Primary

  Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes · CPC title

• C12Q2600/172

  Haplotypes · CPC title

• C12Q1/6888 Primary

  for detection or identification of organisms · CPC title

• A61D19/02

  for artificial insemination · CPC title