Method for isolating polyphenols from olive mill water

Patent metadata

Field	Value
Publication number	US-9420820-B2
Application number	US-201313963120-A
Country	US
Kind code	B2
Filing date	Aug 9, 2013
Priority date	Aug 9, 2013
Publication date	Aug 23, 2016
Grant date	Aug 23, 2016

Abstract

The present invention relate to a highly efficient and novel method, using clean technologies, for obtaining a natural bioactive concentrate that is rich on polyphenols from olive mill water (OMW). The clean technologies integrate centrifugation, a drowning-out crystallization-based separation process, and vacuum evaporation. The method provides a highly-concentrated polyphenol isolate (up to 99% (mass fraction)) from other components presents in OMW, with up to half of the polyphenol content being hydroxytyrosol. The isolated polyphenols exhibit anti-oxidant, anti-microbial, anti-inflammatory, and anti-carcinogenic activities; they can be prepared as solid particles, as an aqueous solution, in an emulsion, or as lipidic-based nanoparticles. The isolated polyphenols can be used in the food industry, cosmetic industry, or pharmaceutical industry.

First claim

What is claimed is:

1. A method for producing a polyphenol-enriched aqueous solution from olive mill water (OMW) comprising the following sequential steps:
(a) obtaining an OMW after olive oil extraction from olive fruits;
(b) pretreating the OMW by removing residual oil, large-sized organic compounds, particles, or soluble molecules from OMW thereby forming a more clear aqueous phase;
(c) concentrating the aqueous phase thereby producing a concentrate;
(d) mixing the concentrate with at least one solvent thereby producing a mixture;
(e) transferring the mixture to a crystallization unit thereby producing a liquid phase and crystals in a solid phase; and
(f) distilling the liquid phase in a distillation unit thereby evaporating the solvent and retaining the polyphenol-enriched aqueous solution.

2. The method of claim 1 , wherein the concentrate of step (c) comprises hydroxytyrosol, oleuropein, caffeic acid, and other minor polyphenols.

3. The method of claim 2 , wherein the final concentration of dissolved solids in the concentrate of step (c) is 40-70% (w/w) containing 60-90% organic matter fraction.

4. The method of claim 1 , wherein the mixing step (d) occurs in one or more mixing modules.

5. The method of claim 1 , wherein solid carbohydrates and solid salts are precipitated out in the crystallization unit of step (e).

6. The method of claim 4 , wherein the one or more mixing modules and the crystallization unit have independently controllable environments including temperature, solvent mass fraction, and numbers of stages.

7. The method of claim 1 , wherein a solid phase comprising a high content of salts and saccharides is crystallized in the crystallization unit.

Assignees

• Univ Tsukuba
• Center Of Biotechnology Of Sfax Univ Of Sfax Mesrs

Inventors

• Nakajima Mitsutoshi
• Neves Marcos
• Isoda Hiroko
• Dammak Ilyes
• Sayadi Sami

Classifications

• A61K36/63

  Oleaceae (Olive family), e.g. jasmine, lilac or ash tree · CPC title

• A23L33/105 Primary

  Plant extracts, their artificial duplicates or their derivatives · CPC title

• A61K2236/00

  Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine · CPC title

• A23L1/3002 Primary

  Human Necessities · mapped topic