Fine particle measurement device, and laminar flow monitoring method and fine particle analysis method in fine particle measurement device

Patent metadata

Field	Value
Publication number	US-9417173-B2
Application number	US-201314418711-A
Country	US
Kind code	B2
Filing date	Jun 5, 2013
Priority date	Aug 7, 2012
Publication date	Aug 16, 2016
Grant date	Aug 16, 2016

Abstract

There is provided a laminar flow monitoring method in a fine particle measurement device, the method including a radiation step of radiating light to a laminar flow, a position detection step of receiving, by a detector, an S polarized component which is separated from scattered light produced from the laminar flow and to which astigmatism is given and acquiring light reception position information of the S polarized component in the detector, and a determination step of determining a state of the laminar flow based on the light reception position information.

First claim

The invention claimed is:

1. A laminar flow monitoring method in a fine particle measurement device, the method comprising:
radiating light to a laminar flow;
receiving, by a detector of which a light reception surface is split into four regions A, B, C, and D, in a lattice shape, an S polarized component which is separated from scattered light produced from the laminar flow and to which astigmatism is given;
acquiring light reception position information of the S polarized component in the detector by calculating a difference D 1 (A−C) between detected values of the region A and the region C not adjacent to the region A; and
determining a state of the laminar flow based on the light reception position information.

2. The laminar flow monitoring method according to claim 1 , wherein the act of acquiring the light reception information further comprises calculating a difference D 2 ((A+C)−(B+D)) between a sum (A+C) of the detected values of the regions A and C and a sum (B+D) of detected values of the regions B and D.

3. The laminar flow monitoring method according to claim 2 , wherein the state of the laminar flow is determined based on the difference D 1 and/or the difference D 2 .

4. The laminar flow monitoring method according to claim 3 , wherein the act of determining the state of the laminar flow further comprises determining the laminar flow to be abnormal when the difference D 1 and/or the difference D 2 deviates from a predetermined range, and determining the laminar flow to be normal when the difference D 1 and/or the difference D 2 is included within the predetermined range.

5. The laminar flow monitoring method according to claim 4 , wherein the laminar flow is determined to be abnormal when an acquisition frequency of the difference D 1 and/or the difference D 2 that deviates from the predetermined range exceeds a predetermined frequency.

6. The laminar flow monitoring method according to claim 5 , wherein the detector comprises a 4-split photodiode.

7. A fine particle analysis method comprising:
detecting light produced from a laminar flow containing fine particles;
obtaining an analysis result of optical characteristics of the fine particles based on intensity information of the light acquired in the light detection step; and
performing laminar flow monitoring, comprising:
radiating light to the laminar flow;
receiving, by a detector of which a light reception surface is split into four regions A, B, C, and D, in a lattice shape, an S polarized component which is separated from scattered light produced from the laminar flow and to which astigmatism is given;
acquiring light reception position information of the S polarized component in the detector by calculating a difference D 1 (A−C) between detected values of the region A and the region C not adjacent to the region A; and
determining whether a state of the laminar flow is normal based on the light reception position information,

wherein only the intensity information acquired while the laminar flow is determined to be normal is extracted and the analysis result is obtained.

8. A fine particle measurement device comprising:
a light radiation unit configured to radiate light to a laminar flow;
a first spectroscopic element configured to separate scattered light produced from the laminar flow into an S polarized component and a P polarized component;
an S polarized detector of which a light reception surface is split into four regions A, B, C, and D, in a lattice shape, the S polarized detector being configured to receive the S polarized component;
an astigmatism element arranged between the first spectroscopic element and the S polarized detector and configured to give astigmatism to the S polarized component; and
determination circuitry configured to receive an output from the S polarized detector, acquire light reception position information of the S polarized component by calculating a difference D 1 (A−C) between detected values of the region A and the region C not adjacent to the region A, and determine a state of the laminar flow based on the light reception position information.

9. The fine particle measurement device according to claim 8 ,
wherein the determination circuitry is further configured to acquire the light reception position information by calculating a difference D 2 ((A+C)−(B+D)) between a sum (A+C) of the detected values of the regions A and C and a sum (B+D) of detected values of the regions B and D.

10. The fine particle measurement device according to claim 9 , wherein the determination circuitry is further configured to determine the state of the laminar flow based on the difference D 1 and/or the difference D 2 .

11. The fine particle measurement device according to claim 10 , wherein the determination circuitry is further configured to determine the laminar flow to be abnormal when the difference D 1 and/or the difference D 2 deviates from a predetermined range, and determines the laminar flow to be normal when the difference D 1 and/or the difference D 2 is included within the predetermined range.

12. The fine particle measurement device according to claim 11 , further comprising:
a display; and
output circuitry,
wherein the output circuitry is configured to cause the display to display information regarding the difference D 1 and/or the difference D 2 .

13. The fine particle measurement device according to claim 12 , wherein the output circuitry is configured to cause the display to display information regarding an abnormal determination of the laminar flow by the determination circuitry.

14. The fine particle measurement device according to claim 13 , wherein the fine particle measurement device is configured to automatically stop when the determination circuitry determines the laminar flow to be abnormal.

15. The fine particle measurement device according to claim 14 , wherein the astigmatism element is a cylindrical lens.

16. The fine particle measurement device according to claim 15 , further comprising:
a second spectroscopic element configured to separate light produced from the laminar flow into the scattered light and fluorescence;
a P polarized light detector configured to detect the P polarized component; and
a fluorescence detector configured to detect the fluorescence.

17. The fine particle measurement device according to claim 16 , further comprising:
a third spectroscopic element configured to separate the fluorescence,
wherein, in the fluorescence detector, a plurality of independent light-receiving elements that detect the fluorescence separated by the third spectroscopic element are arranged.

18. The fine particle measurement device according to claim 8 , wherein the determination circuitry comprises a central processing unit.

19. The fine particle measurement device according to claim 12 , wherein the determination circuitry and the output circuitry comprise a central processing unit.

Assignees

• Sony Corp

Inventors

• Nitta Nao

Classifications

• G01N2011/008

  optical properties · CPC title

• G01N15/1404

  Handling flow, e.g. hydrodynamic focusing · CPC title

• G01N15/1459

  the analysis being performed on a sample stream · CPC title

• G01N2021/6417

  Spectrofluorimetric devices · CPC title

• G01N21/64

  Fluorescence; Phosphorescence · CPC title