Lactic bacterium for texturizing food products selected on basis of phage resistance

Patent metadata

Field	Value
Publication number	US-9416351-B2
Application number	US-201514719058-A
Country	US
Kind code	B2
Filing date	May 21, 2015
Priority date	Jan 28, 2010
Publication date	Aug 16, 2016
Grant date	Aug 16, 2016

Abstract

The present invention relates to a bacterial cell with texturizing property, starter cultures comprising the cell, and dairy products fermented with the starter culture.

First claim

The invention claimed is:

1. A lactic acid bacterium having a mutation in the region between the Pribnow box and the ribosome binding site of the galactokinase (galK) gene, wherein the mutation results in the replacement of C in the sequence TTCAGT (SEQ ID NO:6) downstream from the wildtype Pribnow box with a nucleotide selected from the group consisting of A and G.

2. The lactic acid bacterium according to claim 1 , which belongs to a species selected from the group consisting of Streptococcus thermophilus and Lactobacillus delbrueckii subsp. bulgaricus.

3. The lactic acid bacterium according to claim 1 , which generates a shear stress in fermented milk from 60 to 100 Pa, measured after 12 hours of growth at 37° C., when inoculated in an amount of at least 10E4 cells/mL milk.

4. The lactic acid bacterium according to claim 1 , belonging to a bacterial strain of the species Streptococcus thermophilus selected from the group consisting of CHCC11342 (DSM 22932), CHCC11976 (DSM 22934), and mutants or variants derived from any of these deposited strains having all the identifying characteristics of the deposited strain.

5. The lactic acid bacterium according to claim 4 , wherein the lactic acid bacterium is a mutant or variant derived from one of said deposited strains that generates an increased shear stress in fermented milk compared to the mother strain of said deposited strain, measured after 12 hours of growth at 37° C., when inoculated in an amount of at least 10E4 cells/mL milk.

6. The lactic acid bacterium according to claim 5 , wherein the shear stress in fermented milk is increased by at least about 10% compared to that of the mother strain of said deposited strain.

7. The lactic acid bacterium according to claim 1 , wherein the galK gene further comprises a −10 region having a nucleotide sequence selected from the group consisting of TATGAT (SEQ ID NO:8), TATTAT (SEQ ID NO:9) and TACTAT (SEQ ID NO:10).

8. A composition comprising the lactic acid bacterium of claim 1 .

9. A method for producing a fermented milk product, comprising fermenting a milk substrate with a lactic acid bacterium of claim 1 .

Assignees

• Chr Hansen As

Inventors

• Janzen Thomas
• Christiansen Ditte Ellegaard

Classifications

• C12N9/1205 Primary

  Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases · CPC title

• C12N1/20 Primary

  Bacteria; Culture media therefor · CPC title

• A23Y2220/35

  Human Necessities · mapped topic

• A23Y2220/00

  Human Necessities · mapped topic

• C12N15/746

  for lactic acid bacteria (Streptococcus; Lactococcus; Lactobacillus; Pediococcus; Enterococcus; Leuconostoc; Propionibacterium; Bifidobacterium; Sporolactobacillus) · CPC title