Label-free detection of renal cancer

Patent metadata

Field	Value
Publication number	US-9410949-B2
Application number	US-201113309852-A
Country	US
Kind code	B2
Filing date	Dec 2, 2011
Priority date	Dec 3, 2010
Publication date	Aug 9, 2016
Grant date	Aug 9, 2016

Abstract

Natural and/or synthetic antibodies for specific proteins are adhered to nanoparticles. The nanoparticles are adhered to a substrate and the substrate is exposed to a sample that may contain the specific proteins. The substrates are then tested with surface enhanced Raman scattering techniques and/or localized surface plasmon resonance techniques to quantify the amount of the specific protein in the sample.

First claim

What is claimed is:

1. A surface enhanced Raman scattering sample collection and testing apparatus comprising:
a fibrous substrate; and
a plurality of surface enhanced Raman scattering nanoparticles, wherein the plurality of nanoparticles are metal nanorods, adhered to a surface of the fibrous substrate.

2. The apparatus of claim 1 wherein the fibrous substrate comprises a cellulous substrate.

3. The apparatus of claim 1 wherein the plurality of surface enhanced Raman scattering nanoparticles are gold nanorods.

4. The apparatus of claim 1 wherein the fibrous substrate is a natural fibrous material.

5. The apparatus of claim 1 wherein the fibrous substrate is a synthetic fibrous material.

6. A method of testing a surface for a target substance comprising:
swabbing a surface with a surface enhanced Raman scattering sample collection and testing apparatus comprising:
a fibrous substrate; and; a plurality of surface enhanced Raman scattering nanoparticles, wherein the plurality of nanoparticles are metal nanorods, adhered to a surface of the fibrous substrate; and

performing Raman spectroscopy on a surface enhanced Raman scattering sample collection and testing apparatus.

7. The method of claim 6 wherein the target substance is at least one of a bacteria, a virus, a biological agent, a chemical agent, an explosive, and an explosive residue.

8. The method of claim 6 further comprising:
preparing a solution of nanoparticles;
submerging at least a portion of a cellulose substrate in the prepared solution of nanoparticles; and
removing the portion of the cellulose substrate from the prepared solution of nanoparticles.

Assignees

• Singamaneni Srikanth
• Kharasch Evan
• Morrissey Jeremiah J
• Lee Chang Hee
• Univ Washington

Inventors

• Singamaneni Srikanth
• Kharasch Evan
• Morrissey Jeremiah J
• Lee Chang Hee

Classifications

• G01N33/57525

  of the liver or pancreas · CPC title

• G01N21/554

  detecting the surface plasmon resonance of nanostructured metals, e.g. localised surface plasmon resonance · CPC title

• G01N33/54346 Primary

  Nanoparticles · CPC title

• G01N21/658

  enhancement Raman, e.g. surface plasmons · CPC title

• G01N33/54373

  involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings · CPC title